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Rabbit Anti-CD163 Polyclonal Antibody#abs137712

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Description

Catalog-specification	Delivery time	USD price
abs137712-100ug	1-2 Weeks	301.0
abs137712-50ug	1-2 Weeks	201.0

Overview
catalog	abs137712
Other names	The CD163 antigen, also known as C163A_HUMAN or Hemoglobin scavenger receptor, is a molecule found on macrophages and is associated with M130 antigen precursor. It is a scavenger receptor cysteine rich type 1 protein that plays a role in binding and scavenging hemoglobin. Additionally, it is involved in various physiological processes. Another name for CD163 is M130, and it is encoded by genes such as SCARI1 and OTTHUMP00000238617. There are also other related gene variants such as OTTHUMP00000238618, OTTHUMP00000238619, and OTTHUMP00000238620. Soluble CD163, often referred to as sCD163, is a form of CD163 that can be detected in the bloodstream.
Source	Rabbit
Specificity	Endogenous levels of total CD163 can be detected using the CD163 Antibody. This antibody is designed to specifically target CD163 and is able to identify its various forms present in biological samples. With this antibody, researchers can monitor the levels of CD163 expression in a variety of tissues and experimental conditions. Its specificity and sensitivity make this antibody a valuable tool for investigating the physiological function and regulation of CD163. By detecting endogenous CD163 levels, researchers can gain insights into the role this protein plays in various biological processes.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Dog;Horse
Antigen	CD163
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human CD163.
MW	125 kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized in the peptide affinity chromatography process to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain product quality, it is advised to store the item at a temperature of -20 °C for a duration of one year. It is crucial to avoid subjecting the product to repeated cycles of freezing and thawing.
Storage buffer	The storage conditions for Rabbit IgG are as follows: it should be stored at -20°C. The IgG is formulated in phosphate buffered saline with a pH of 7.4. It contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. This product is stable for a period of 12 months from the date of receipt.
Target
Background	Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
Tissue specificity	Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.
Posttranslational modification	A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.Phosphorylated.
Celluar localization	Extracellular region or secreted;Plasma Membrane;
UniPort	Q86VB7