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Rabbit Anti-CCND2 Polyclonal Antibody#abs136128

Description

Catalog-specification	Delivery time	USD price
abs136128-100ug	1-2 Weeks	301.0
abs136128-50ug	1-2 Weeks	201.0

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Overview
catalog	abs136128
Description	This gene encodes a protein that is a member of the cyclin family, which is highly conserved and exhibits periodic changes in protein abundance throughout the cell cycle. Cyclins play a role in regulating CDK kinases. Different cyclins have distinct expression and degradation patterns, contributing to the precise timing of each stage of cell division. This particular cyclin forms a complex with CDK4 or CDK6 and acts as a regulatory subunit, essential for the G1/S transition of the cell cycle. It is also involved in the phosphorylation of the tumor suppressor protein Rb. Studies using mouse models lacking this gene's homolog suggest its vital functions in the proliferation of ovarian granulosa and germ cells. Furthermore, elevated expression of this gene has been observed in ovarian and testicular tumors.
Other names	Cyclin D2, also known as CCND2 or CCND2_HUMAN, is a crucial protein involved in regulating the cell cycle. It plays a vital role in driving the cell cycle progression from G1 to S phase. Additionally, this protein is specific to G1/S phase and is often referred to as G1/S-specific cyclin-D2 or G1/S specific cyclin D2. The gene encoding cyclin D2 is located on human chromosome 12 and is alternatively known as MPPH3 or KIAK0002. Notably, this protein is highly conserved across species and is expressed in a wide range of tissues. Furthermore, mutations in this gene have been linked to various human cancers, making it a promising target for cancer therapies. Other aliases for cyclin D2 include MGC102758.
Source	Rabbit
Specificity	The CCND2 Antibody is designed to detect total CCND2 at levels that are naturally present within cells.
Species Reactivity	Human;Mouse;Rat
Antigen	CCND2
Application	Here are some rearranged versions of the original content: - For Western blotting, the recommended dilution range is 1:500 to 1:2000. When performing immunohistochemistry, it is suggested to dilute the antibody at 1:50 to 1:200. For immunofluorescence and immunocytochemistry, the optimal dilution range is 1:100 to 1:500. If using the antibody for ELISA with a peptide substrate, the recommended dilution is between 1:20000 and 1:40000. - When using this antibody for Western blot analysis, it is best to dilute it between 1:500 and 1:2000. For immunohistochemistry, the suggested dilution range is 1:50 to 1:200, while for immunofluorescence and immunocytochemistry, it is recommended to use a dilution between 1:100 and 1:500. ELISAs with peptide substrates require a dilution of 1:20000 to 1:40000. - The optimal dilution range for Western blotting with this antibody is 1:500 to 1:2000. Immunohistochemistry is best performed with a dilution of 1:50 to 1:200, whereas immunofluorescence and immunocytochemistry require a dilution range of 1:100 to 1:500. For ELISA with a peptide substrate, the recommended dilution is between 1:20000 and 1:40000.
Immunogen	A synthesized peptide derived from human CCND2.
MW	33kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).
Posttranslational modification	Polyubiquitinated by the SCF(FBXL2) complex, leading to proteasomal degradation.
Celluar localization	Cytosol;Nucleus;
UniPort	P30279