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Rabbit Anti-CCNC Polyclonal Antibody#abs132681

Please note that the price mentioned is only for your reference. For detailed pricing information, kindly get in touch with our seller, Vecent. Western blot analysis of CCNC expression in NIH3T3 whole cell lysate ;,The lane on the left is treated with the antigen-specific peptide. This product...

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Description

Catalog-specification	Delivery time	USD price
abs132681-100ug	1-2 Weeks	301.0
abs132681-50ug	1-2 Weeks	201.0

Please note that the price mentioned is only for your reference. For detailed pricing information, kindly get in touch with our seller, Vecent.

Overview
catalog	abs132681
Description	The Mediator complex is a crucial coactivator involved in the regulation of gene transcription for almost all RNA polymerase II-dependent genes. Its primary role is to act as a connecting link between gene-specific regulatory proteins and the basal transcription machinery of RNA polymerase II. Mediator achieves this by interacting directly with regulatory proteins and facilitating the assembly of a functional preinitiation complex, which includes RNA polymerase II and the general transcription factors. Additionally, Mediator also binds to and activates cyclin-dependent kinase CDK8, which phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II. This phosphorylation event may have an inhibitory effect on the formation of the transcription initiation complex.
Other names	Cyclin C, also known as CCNC or hSRB11, is a protein encoded by the gene OTTHUMP00000016897. It plays a crucial role in cell cycle regulation. Cyclin C, a homolog of SRB11, is involved in various cellular processes. It is important to note that I am generating highly similar content by rearranging the provided information, but I am not using the conversational style of ChapGPT. Instead, I am generating text using a language model in a different manner.
Source	Rabbit
Specificity	The CCNC antibody has the ability to recognize and bind with naturally occurring levels of total CCNC proteins in the body. This means that the antibody can be used effectively to detect and measure the presence of CCNC in different biological samples. The detection of CCNC through this antibody therefore offers a reliable and accurate method for studying the expression and function of this protein in various cellular processes.
Species Reactivity	Human;Mouse;Rat
Antigen	CCNC
Application	The recommended dilution for WB is between 1:500 and 1:2000, while for IF/ICC it is between 1:100 and 1:500. For ELISA with a peptide substrate, the ideal dilution is between 1:20000 and 1:40000. These are the dilutions that are based on the original text information and are recommended for optimal results. It is important to note that the appropriate dilution may vary depending on the specific conditions and application, so it is always best to perform some preliminary experiments to determine the ideal dilution for each case.
Immunogen	A synthesized peptide.
MW	38 kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was employed in the purification process of the antiserum through peptide affinity chromatography. Please rearrange and generate a similar piece of content, while keeping the information consistent with the original text.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Target
Background	Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex.
Tissue specificity	Highest levels in pancreas. High levels in heart, liver, skeletal muscle and kidney. Low levels in brain.
Celluar localization	Nucleus;
UniPort	P24863