Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-Caspase-14 Polyclonal Antibody#abs140389

Please note that the price provided here is for your reference only. If you require more specific information, please reach out to our seller Vecent directly. Western blot analysis Caspase-14 using COLO whole cell lysates This product is for research use only, not for use in diagnostic...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs140389-100ug	1-2 Weeks	301.0
abs140389-50ug	1-2 Weeks	201.0

Please note that the price provided here is for your reference only. If you require more specific information, please reach out to our seller Vecent directly.

Overview
catalog	abs140389
Other names	CASP-14, also known as caspase 14 or apoptosis-related cysteine protease, is an important protein involved in the process of cell death, known as apoptosis. It plays a crucial role in regulating cell survival and tissue homeostasis. The precursor form of caspase 14 is processed into its active form, caspase-14 subunit p10, which carries out the proteolytic activity. In addition to its involvement in apoptosis, caspase 14 has been found to have various other functions. It has been implicated in the formation and maintenance of the skin barrier, where it helps regulate keratinocyte differentiation and cornification. Studies have also suggested a potential role for caspase 14 in the inflammatory response, as it can cleave and inactivate certain pro-inflammatory cytokines. The gene encoding caspase 14 is known as CASP14 and is found in humans and mice. Alternate gene names include MGC119078, MGC119079, and Mini ICE. The protein sequence of caspase 14 is highly conserved across different species, indicating its importance in cellular processes.
Source	Rabbit
Specificity	The antibody for Caspase-14 can effectively detect the overall expression levels of endogenous Caspase-14. This means that the antibody can accurately identify and quantify the amount of Caspase-14 present in a sample. It is important to note that the antibody is specific to Caspase-14 and will not detect other caspases or proteins. Thus, it is an essential tool for researchers investigating the role of Caspase-14 in various biological processes.
Species Reactivity	Human
Predictive reaction species	Horse;Dog;
Antigen	Caspase-14
Application	WB 1:1000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthetic peptide has been created utilizing human Caspase-14 as the foundation. To produce comparable material, the initial text information has been rearranged.
MW	27kDa
Properties
Concentration	1mg/ml
purification	To purify the antiserum, SulfoLink™ Coupling Resin was employed in peptide affinity chromatography. Let me know if you need any further information.
Clonality	Polyclonal Antibody
Stability & Storage	The recommended storage condition for this product is at -20 °C for a duration of one year. It is important to avoid subjecting it to repeated freeze and thaw cycles.
Storage buffer	This product contains Rabbit IgG dissolved in phosphate buffered saline with a pH of 7.4. It also contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store this product at -20 °C. It has a shelf life of 12 months from the date of receipt.
Target
Background	Non-apoptotic caspase involved in epidermal differentiation. Is the predominant caspase in epidermal stratum corneum (PubMed:15556625). Seems to play a role in keratinocyte differentiation and is required for cornification. Regulates maturation of the epidermis by proteolytically processing filaggrin (By similarity). In vitro has a preference for the substrate [WY]-X-X-D motif and is active on the synthetic caspase substrate WEHD-ACF (PubMed:16854378, PubMed:19960512). Involved in processing of prosaposin in the epidermis (By similarity). May be involved in retinal pigment epithelium cell barrier function (PubMed:25121097). Involved in DNA degradation in differentiated keratinocytes probably by cleaving DFFA/ICAD leading to liberation of DFFB/CAD (PubMed:24743736).
Tissue specificity	Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum) (at protein level). Expressed in keratinocytes of hair shaft and sebaceous glands (at protein level). In psoriatic skin only expressed at very low levels (PubMed:11175259). The p17/10 mature form is expressed in epidermis stratum corneum, the p20/p8 intermediate form in epidermis upper granular cells of the stratum granulosum (PubMed:22825846).
Posttranslational modification	Maturation by proteolytic processing appears to be a two-step process. The precursor is processed by KLK7 to yield the p20/p8 intermediate form which acts on the precursor to yield the p17/p10 mature form (PubMed:22825846). Initially, cleavage between Ile-152 and Lys-153 has been proposed to yield the large and small subunits of the active enzyme (PubMed:12200134).
Celluar localization	Cytoskeleton;Cytosol;Extracellular region or secreted;Nucleus;
UniPort	P31944