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Rabbit Anti-CAD Polyclonal Antibody#abs137135

Description

Catalog-specification	Delivery time	USD price
abs137135-100ug	1-2 Weeks	301.0
abs137135-50ug	1-2 Weeks	201.0

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Overview
catalog	abs137135
Other names	CAD protein, also known as aspartate carbamoyltransferase or aspartate transcarbamylase, is a multifunctional protein that plays a key role in the biosynthesis of pyrimidine nucleotides. This trifunctional protein is a fusion of carbamoyl phosphate synthetase 2 (CPSase), aspartate transcarbamylase (ATCase), and dihydroorotase (DHOase) domains. CAD protein is involved in the first three steps of the de novo biosynthesis of pyrimidine nucleotides, which begins with the formation of carbamoyl phosphate. This reaction is catalyzed by CPSase, which is present in the CAD protein complex. The second step involves the transfer of carbamoyl phosphate to aspartate by ATCase, also present in CAD protein. Finally, DHOase catalyzes the conversion of dihydroorotate to orotate, which can be further converted to uridine monophosphate (UMP), a precursor for the synthesis of all pyrimidine nucleotides. Interestingly, CAD protein is highly conserved across species, with homologs found in bacteria, plants, and animals. It is also highly regulated, with allosteric regulation by ATP and UTP, as well as feedback inhibition by the end product UMP. Dysfunctional CAD protein is associated with various genetic diseases, including hereditary orotic aciduria and N-carbamylglutamate synthase deficiency. In conclusion, CAD protein is a critical enzyme complex that plays a vital role in pyrimidine nucleotide biosynthesis. Its multifunctional nature, conservation, and regulation highlight its importance in maintaining cellular homeostasis.
Source	Rabbit
Specificity	The CAD Antibody has the ability to identify total CAD present in the body naturally. This means that it can recognize and bind to CAD proteins in order to provide an accurate reading about their concentration.
Species Reactivity	Human
Antigen	CAD
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human CAD.
MW	250 kDa
Properties
Concentration	1mg/ml
purification	The SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography to purify the antiserum. This process resulted in a highly purified product with similar properties to the original substance.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is recommended to store it at a temperature of -20°C for a period of one year. It is important to note that repeated freeze and thaw cycles should be avoided to prevent any potential damage to the product. Therefore, it is crucial to handle the product carefully while storing and using it.
Storage buffer	This Rabbit IgG solution comes in phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended that you store the solution at -20 °C for up to 12 months. It's important to note that the solution is stable from the date of receipt, and should not be used after this period. Make sure to follow the guidance on product handling and storage to ensure optimal results.
Target
Background	This protein is a "fusion" protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).
Tissue specificity	Transcriptionally repressed following hypoxia by HIF1A.
Posttranslational modification	Activated by MAP kinase (Erk1/2) phosphorylation just prior to the S phase of the cell cycle, when the demand for pyrimidine nucleotides is greatest, and down-regulated as the cells emerge from S phase by protein kinase A (PKA) phosphorylation. Phosphorylation at Ser-1859 by RPS6KB1 downstream of MTOR promotes oligomerization and stimulates dihydroorotase activity. Phosphorylation at Ser-1406 reduces sensitivy to feedback inhibition by UTP.
Celluar localization	Cytosol;Extracellular region or secreted;Nucleus;
UniPort	P27708