Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-ATPG Polyclonal Antibody#abs140883

Please note that the price mentioned above is for your reference only. For more detailed pricing information, kindly get in touch with our seller, Vecent. It is important to contact Vecent for accurate pricing details. Western blot analysis of extracts from HeLa cells and Jurkat cells, using...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs140883-100ug	1-2 Weeks	301.0
abs140883-50ug	1-2 Weeks	201.0

Overview
catalog	abs140883
Other names	The ATP synthase gamma chain, also known as ATP synthase subunit gamma or ATP synthase subunit gamma, mitochondrial, is an important component of the ATP synthase complex located in the mitochondria. This protein, encoded by the ATP5C1 gene, is essential for ATP synthesis in the presence of a proton-motive force that is generated by the electron transport chain. The gamma subunit of the F1-ATPase complex plays a critical role in the rotation of the enzyme, which is necessary for ATP synthesis. It is a highly conserved protein across different species and variations in its expression have been linked to various diseases, including mitochondrial disorders and cancer. In summary, the mitochondrial ATP synthase gamma subunit 1 is a crucial component for the generation of energy in the form of ATP through the electron transport chain.
Source	Rabbit
Specificity	The ATPG antibody is capable of identifying the existing amounts of overall ATPG within an organism. It does not require any external manipulation for detection and provides an accurate representation of the levels present.
Species Reactivity	Human;Mouse;Rat
Antigen	ATPG
Application	The recommended dilution range for Western Blot is 1:500~1:1000, for Immunofluorescence or Immunocytochemistry it is 1:100-1:500, and for ELISA with peptide it is 1:20000-1:40000. To ensure accuracy, it is important to maintain the original text information while rearranging it.
Immunogen	A synthesized peptide.
MW	33 KD
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized in the peptide affinity chromatography process to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure the integrity of the product, it is advised to store it at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to multiple freeze/thaw cycles, as this may affect its quality.
Storage buffer	-20°CIgG，pH7.4，150mMNaCl，0.02%50%。，12。，，。：-20°CIgG ：，pH7.4，150mMNaCl，0.02%50%。：-20°C ：12。
Target
Background	Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F1 domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha3beta3. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
Tissue specificity	Isoform Heart is expressed specifically in the heart and skeletal muscle, which require rapid energy supply. Isoform Liver is expressed in the brain, liver and kidney. Isoform Heart and Isoform Liver are expressed in the skin, intestine, stomach and aorta.
Celluar localization	Extracellular region or secreted;Mitochondrion;
UniPort	P36542