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Rabbit Anti-ASPM Polyclonal Antibody#abs139584

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Description

Catalog-specification	Delivery time	USD price
abs139584-100ug	1-2 Weeks	301.0
abs139584-50ug	1-2 Weeks	201.0

Overview
catalog	abs139584
Source	Rabbit
Specificity	The ASPM Antibody can be used to measure the total ASPM levels naturally present in the body.
Species Reactivity	Human
Predictive reaction species	Horse
Antigen	ASPM
Application	Here are some alternatives: - If you're planning to use Western Blotting, you should dilute your primary antibody between 1:1000 and 1:3000. For Immunohistochemistry, a dilution of 1:50 to 1:200 is recommended. Finally, if you're planning to perform an ELISA using a peptide as your antigen, a dilution of 1:20000 to 1:40000 should be sufficient. - Depending on the assay you're planning to perform, you'll need to dilute your primary antibody accordingly. For Western Blotting, the recommended dilution range is between 1:1000 and 1:3000. For Immunohistochemistry, a dilution of 1:50 to 1:200 is appropriate. If you're planning to do an ELISA using a peptide as your antigen, diluting your primary antibody between 1:20000 and 1:40000 should work well. - The optimal dilution range for primary antibodies varies depending on the assay you're performing. For Western Blotting, the recommended dilution is between 1:1000 and 1:3000. If you're doing Immunohistochemistry, diluting your primary antibody between 1:50 and 1:200 is advised. When using a peptide in an ELISA, a dilution of 1:20000 to 1:40000 should yield good results. - When preparing your primary antibody for assay, it's important to dilute it appropriately. For Western Blotting, a dilution range of 1:1000 to 1:3000 is recommended. Immunohistochemistry requires a dilution of 1:50 to 1:200 for the primary antibody. If you're using a peptide in an ELISA, a dilution of 1:20000 to 1:40000 is recommended for the primary antibody. - The success of your assay depends on the correct dilution of your primary antibody. For Western Blotting, aim for a dilution range of 1:1000 to 1:3000. Immunohistochemistry requires a dilution of 1:50 to 1:200 for the primary antibody. If you're using a peptide in an ELISA, the recommended dilution for the primary antibody is between 1:20000 and 1:40000.
Immunogen	A synthesized peptide derived from human ASPM.
MW	410 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, peptide affinity chromatography was employed to purify the antiserum. A highly similar content can be created by simply rephrasing the original text as follows: The antiserum was subjected to peptide affinity chromatography, which involved the use of SulfoLink™ Coupling Resin for purification.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure long-term storage, maintain a temperature of -20 °C for one year without subjecting the samples to repetitive freeze/thaw cycles.
Storage buffer	The provided information is related to Rabbit IgG stored in phosphate buffered saline, pH 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store it at a temperature of -20 °C. The stability of Rabbit IgG is guaranteed for 12 months from the date of receipt.
Target
Background	The protein is involved in the regulation and coordination of various processes during mitosis, particularly in ensuring the proper functioning of the mitotic spindle. Its main role is to regulate the dynamics of microtubules at the spindle poles, which includes controlling spindle orientation, density of astral microtubules, and the flux of microtubules towards the poles. To carry out this function effectively, it forms a complex with two other proteins called KATNA1 and KATNB1, collectively known as the katanin complex. This association enhances the ability of KATNA1 to sever the microtubule lattice. By recruiting the katanin complex to the microtubules, it facilitates the severing of microtubules. In addition, it can also inhibit the growth of microtubules at their minus ends, and interestingly, this inhibition can be further strengthened by the presence of the katanin complex. This protein may also play a specific role in regulating neurogenesis, the process of generating new neurons in the nervous system.
Celluar localization	Cytoskeleton;Nucleus;
UniPort	Q8IZT6