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Rabbit Anti-ALKBH3 Polyclonal Antibody#abs132828

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Description

Catalog-specification	Delivery time	USD price
abs132828-100ug	1-2 Weeks	301.0
abs132828-50ug	1-2 Weeks	201.0

Overview
catalog	abs132828
Description	This enzyme is known as an alkyl DNA dioxygenase and is responsible for repairing DNA damage caused by alkylation, specifically targeting 1-methyladenine and 3-methylcytosine. It achieves this through a process called oxidative demethylation. Notably, this dioxygenase exhibits a strong preference for single-stranded DNA structures. Additionally, there is evidence to suggest that it may also act on RNA molecules. In order to function, this enzyme requires the presence of molecular oxygen, alpha-ketoglutarate, and iron. By utilizing these essential cofactors, the alkyl DNA dioxygenase can effectively carry out its demethylation repair mechanism.
Other names	ABH3, also known as ALKBH3 or DEPC1, is an alkylated DNA repair protein which belongs to the alpha-ketoglutarate-dependent dioxygenase alkB homolog family. It is a homolog of the E. coli alkylation repair protein alkB and plays a vital role in repairing DNA damage caused by different types of alkylating agents. The gene encoding ABH3 is located on human chromosome 16 and is expressed in a wide range of tissues. ABH3 has been implicated in the development of prostate cancer and is considered to be a potential marker for this disease. Besides, it has been shown to be involved in regulating RNA methylation and DNA demethylation, suggesting a broader role in epigenetic regulation. In summary, ABH3 serves as an essential component of the DNA repair machinery and has emerged as a promising target for cancer therapy.
Source	Rabbit
Specificity	The detection of endogenous levels of total ALKBH3 can be achieved using the ALKBH3 antibody. For accurate results, it is important to use an antibody that specifically recognizes ALKBH3 and can detect its total expression.
Species Reactivity	Human;Mouse
Predictive reaction species	Chicken;Rabbit;Pig;Dog;Sheep;Horse;Bovine
Antigen	ALKBH3
Application	The dilution ratios for Western Blot (WB) range from 1:500 to 1:2000, for immunohistochemistry (IHC) range from 1:50 to 1:200, and for ELISA (peptide) range from 1:20000 to 1:40000.
Immunogen	A synthesized peptide.
MW	33 kDa
Properties
Concentration	1mg/ml
purification	Through the utilization of SulfoLink™ Coupling Resin, the antiserum underwent purification via peptide affinity chromatography. This process resulted in the product being thoroughly refined and highly effective.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Target
Background	Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A) (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:26863196, PubMed:26863410). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation (PubMed:12486230, PubMed:12594517, PubMed:16174769). Has a strong preference for single-stranded DNA (PubMed:12486230, PubMed:12594517, PubMed:16174769). Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3 (PubMed:22055184). Also acts on RNA (PubMed:12594517, PubMed:16174769, PubMed:26863196, PubMed:26863410, PubMed:16858410). Demethylates N1-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons (PubMed:26863196, PubMed:26863410). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:22055184, PubMed:16858410).
Tissue specificity	Ubiquitous. Detected in heart, pancreas, skeletal muscle, thymus, testis, ovary, spleen, prostate, small intestine, peripheral blood leukocytes, urinary bladder and colon.
Celluar localization	Cytosol;Mitochondrion;Nucleus;
UniPort	Q96Q83