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Rabbit Anti-5-HT-4 Polyclonal Antibody#abs133805

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Description

Catalog-specification	Delivery time	USD price
abs133805-100ug	1-2 Weeks	301.0
abs133805-50ug	1-2 Weeks	201.0

Overview
catalog	abs133805
Other names	The 5-HT4 receptor, also known as the 5 hydroxytryptamine serotonin receptor 4, is a G protein-coupled receptor that binds with the neurotransmitter serotonin. Other names that refer to this receptor include 5-HT-4, 5H4, HTR 4, and serotonin 4 receptor. The cardiac 5-HT4 receptor is specific to the heart and has been studied for its role in regulating heart rate and contractility. Serotonin, which is produced and released by neurons in the brain, plays a key role in the regulation of mood, appetite, and sleep, among other functions. The 5-HT4 receptor has been targeted by pharmaceutical companies for the development of drugs to treat gastrointestinal disorders and depression.
Source	Rabbit
Specificity	The levels of total 5-HT-4 present in the body can be effectively detected using the 5-HT-4 Antibody. This antibody has the capacity to detect the endogenous levels of total 5-HT-4 accurately, enabling researchers to study the functions and implications of this protein.
Species Reactivity	Human;Mouse;Rat
Antigen	5-HT-4
Application	WB 1:500~1:1000, IF/ICC 1:100-1:500, and ELISA (peptide) 1:20000-1:40000 are the recommended dilution ranges for various biological assays commonly used in research. These dilution ranges ensure optimal signal detection while minimizing background noise. For Western Blot (WB), the suggested dilution ratio is between 1:500 and 1:1000, meaning the primary antibody is diluted 500 to 1000 times before use. Similarly, for Immunofluorescence (IF) or Immunocytochemistry (ICC), the recommended dilution ratio ranges from 1:100 to 1:500, implying a dilution of the primary antibody between 100 to 500 times. Lastly, for Enzyme-Linked Immunosorbent Assay (ELISA) using peptides as antigens, the suggested dilution range is 1:20000 to 1:40000, indicating the primary antibody should be diluted 20000 to 40000 times. These dilution guidelines are essential to achieve accurate and specific results in various experimental techniques.
Immunogen	A synthesized peptide.
MW	43 KD
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was used for peptide affinity chromatography to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain its quality, please store at a temperature of -20 °C for a duration of one year. It is crucial to avoid subjecting the product to multiple freeze and thaw cycles.
Storage buffer	The rabbit IgG is dissolved in phosphate buffered saline with a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide and 50% glycerol. It should be stored in a freezer at -20 °C and can remain stable for up to 12 months from the date of receipt.
Target
Background	This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase.
Tissue specificity	Isoform 5-HT4(A) is expressed in ileum, brain, and atrium, but not in the ventricle.
Celluar localization	Endosome;Plasma Membrane;
UniPort	Q13639