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Phospho-VAMP4 (Ser30) Rabbit Polyclonal Antibody#abs139924

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Description

Catalog-specification	Delivery time	USD price
abs139924-100ug	1-2 Weeks	301.0
abs139924-50ug	1-2 Weeks	201.0

Overview
catalog	abs139924
Other names	VAMP 24 is also known as VAMP-4 or VAMP4 protein. It is a vesicle associated membrane protein 4 that plays a crucial role in cell membrane trafficking. VAMP 24, encoded by the VAMP4_HUMAN gene, is involved in the fusion of transport vesicles with target membranes. It facilitates the release of cargo molecules from vesicles, allowing them to be transported to their specific destinations within the cell. VAMP 24 isoforms, CRA a and CRA b, are alternative forms of the protein that may have different functions or regulation mechanisms. These isoforms may be expressed in different cell types or under specific conditions, contributing to the versatility of VAMP 24 in diverse cellular processes. The presence of VAMP 24 in vesicles suggests its involvement in intracellular protein transport and secretion pathways. By participating in these processes, VAMP 24 enables the precise delivery of proteins to their intended locations, ensuring the proper functioning of various cellular activities. Understanding the molecular characteristics of VAMP 24 and its isoforms provides valuable insights into the intricate mechanisms of cellular transport and membrane fusion. Further research on VAMP 24 can uncover its specific roles in different cells and shed light on its contribution to fundamental biological processes. By deciphering the functions of VAMP 24 and its isoforms, scientists can potentially develop targeted therapies for diseases related to membrane trafficking dysregulation.
Source	Rabbit
Specificity	The detection of endogenous levels of VAMP4 is possible with the VAMP4 (Phospho-Ser30) Antibody, but only when the protein is phosphorylated specifically at Ser30. By utilizing this antibody, researchers can accurately identify and study the phosphorylation state of VAMP4 at Ser30.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Rabbit;Sheep;Dog;Bovine;Horse;
Antigen	VAMP4
Application	The recommended dilutions for detecting target proteins through Western blot range from 1:1000-3000, while in immunohistochemistry, dilutions between 1:50-1:200 are suggested. For peptide-based ELISA assays, a dilution of 1:20000-1:40000 is optimal. These dilutions have been tailored to ensure the highest detection sensitivity and specificity for the target proteins of interest. Please note that these dilutions may need to be adjusted depending on the specific experimental conditions and the quality of the primary antibody being used.
Immunogen	A peptide that has been synthesized is derived from human VAMP4, which has been phosphorylated on Serine 30. To craft a highly similar piece of content, the primary focus should remain based on the original text's information. However, it must be ensured that the wording and sentence structure remain entirely different from what has already been mentioned.
MW	16KD
Properties
Concentration	1mg/ml
purification	The process for obtaining the antibody involved purifying rabbit serum and utilizing sequential chromatography on both phospho- and non-phospho-peptide affinity columns. By doing so, a highly specific and purified antibody was obtained.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain optimal conditions, it is recommended to store the product at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing. Ensure the storage environment is consistent to preserve the product's quality.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. May be a marker for this sorting pathway that is critical for remodeling the secretory response of granule.
Celluar localization	Endosome;Golgi apparatus;Lysosome;Plasma Membrane;
UniPort	O75379