Phospho-SHP-2 (Tyr542) Rabbit Polyclonal Antibody#abs131037

abs131037-50ug

1-2 Weeks

201

abs131037-100ug

1-2 Weeks

301

Description

SHP-2 is a protein phosphatase that contains an SH2 domain and is found throughout the body. This phosphatase plays a significant role in signaling pathways that are activated by growth factors, cytokines, hormones, antigens and extracellular matrices. These pathways control various cellular processes, such as growth, differentiation, migration and apoptosis.

Other names

Shp2, also known as protein tyrosine phosphatase non receptor type 11, is a protein tyrosine phosphatase that plays a vital role in many biological processes. It is encoded by the PTPN11 gene and is expressed in various tissues throughout the body.
Shp2 has several other aliases, including PTP-1D, PTP-2C, PTP1D, PTP2C, and SAP2. Additionally, it is sometimes referred to as SH-PTP2, SH-PTP3, SH2 domain containing protein tyrosine phosphatase 2, or Syp.
One of Shp2's key functions is its ability to regulate signaling pathways that control cell growth, differentiation, and migration. It accomplishes this through its catalytic activity, which allows it to remove phosphate groups from specific tyrosine residues on proteins.
Shp2 has also been implicated in various disease states, including cancer, developmental disorders, and cardiovascular disease. Thus, understanding its function and regulation has significant clinical implications.
In conclusion, Shp2 is a multifaceted protein that is crucial for proper cellular function and may serve as a therapeutic target for certain diseases. Its various aliases reflect its many roles in different biological contexts.

Source

Rabbit

Specificity

The antibody known as Phospho-SHP-2 (Tyr542) is specifically designed to identify the presence of SHP-2 at Tyrosine 542 when it is in a phosphorylated state. This antibody is capable of detecting the endogenous levels of SHP-2, but only when it is in a phosphorylated form. It is important to note that this antibody does not react with other forms of SHP-2, and is highly specific to the Tyr542 residue. Through the use of this antibody, researchers are able to gain insights into the activity and function of SHP-2 in cells, and in turn, develop new therapies for diseases and disorders.

Predictive reaction species

Chicken;Rabbit;Pig;Dog;Sheep;Horse;Bovine

Application

The recommended dilution range for this antibody is WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, and ELISA (peptide) 1:20000-1:40000. You can achieve optimal results by using the antibody within these dilution ranges. We strongly suggest testing the antibody with a range of sample concentrations to determine the optimal dilution that provides the highest signal to noise ratio. By doing so, you will ensure a highly specific and sensitive detection of your target protein of interest. Additionally, incorporating appropriate controls will help to ensure the validity of your experimental results.

A peptide synthesized from human SHP-2 has been developed that is focused on the phosphorylation site of Tyrosine 542. The derived peptide closely mimics the natural peptide in structure and function, and offers the potential to serve as a targeted therapy for diseases and disorders associated with aberrant SHP-2 activity. This innovative approach to drug development holds great promise for improving patient outcomes and advancing our understanding of complex molecular mechanisms governing cellular behavior. With ongoing research and development, we hope to unlock new treatments that can help address some of the most pressing medical challenges of our time.

Properties

1mg/ml

Purification

Sequential chromatography was used to purify the antibody from rabbit serum, specifically through affinity purification on phospho- and non-phospho-peptide affinity columns.

Clonality

Polyclonal Antibody

For a duration of one year, it is recommended to store the item in a temperature of -20 °C. It is important to avoid subjecting it to multiple freeze/thaw cycles.

Storage buffer

This product contains rabbit IgG in a solution of phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store this product at a temperature of -20 °C for maximum stability, and it will remain stable for a period of 12 months from the date of receipt.

Target

Background

 Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway (PubMed:28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed:28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulatation of its RhoA binding activity. Dephosphorylates CDC73 (PubMed:26742426).

Tissue specificity

Widely expressed, with highest levels in heart, brain, and skeletal muscle.

Celluar localization

Cytosol;Mitochondrion;Nucleus;

Q06124