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Phospho-ROS (Tyr2274) Rabbit Polyclonal Antibody#abs140015

Please note that the price mentioned above is only for your reference. To obtain the actual price and more detailed information, please contact our seller, Vecent. Western blot analysis ROS (Phospho-Tyr2274) using IL-4 treated HeLa whole cell lysates This product is for research use only, not...

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Description

Catalog-specification	Delivery time	USD price
abs140015-100ug	1-2 Weeks	301.0
abs140015-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is only for your reference. To obtain the actual price and more detailed information, please contact our seller, Vecent.

Overview
catalog	abs140015
Other names	Here are a few variants of the original content: - The ROS1 gene encodes a receptor tyrosine kinase that is sometimes called c-Ros or proto-oncogene c-Ros-1. It belongs to the ROS family of transmembrane tyrosine-specific protein kinases and is involved in the regulation of cell growth, differentiation, and survival. Aberrant activation of ROS1 by gene fusions or mutations has been implicated in various types of cancer, including non-small cell lung cancer and cholangiocarcinoma. - ROS is a proto-oncogene that codes for a tyrosine kinase receptor known as c-ROS-1. This protein plays a role in cell signaling pathways that control cell growth and proliferation. Mutations or rearrangements of the ROS gene can lead to the activation of its kinase activity and contribute to the development of cancer. ROS1 fusions have been identified in a subset of lung adenocarcinomas and are considered a potential target for therapy with tyrosine kinase inhibitors. - The ROS1 proto-oncogene encodes a transmembrane tyrosine-specific protein kinase that is expressed in various tissues and cellular contexts. It has been implicated in the regulation of cell adhesion, migration, and survival through different downstream effectors and signaling pathways. ROS1 mutations or gene fusions have been identified in a range of malignancies, such as glioblastoma, ovarian cancer, and inflammatory myofibroblastic tumor. Targeting ROS1 activation has emerged as a promising therapeutic strategy for patients with ROS1-altered tumors.
Source	Rabbit
Specificity	The ROS (Phospho-Tyr2274) Antibody is able to detect the presence of ROS, but only when it has undergone phosphorylation at the Tyr2274 site. This antibody works by specifically targeting endogenous levels of ROS that have undergone this specific modification. It is important to note that without this phosphorylation, detection of ROS levels may not be possible with this antibody.
Species Reactivity	Human;Mouse;Rat
Antigen	ROS
Application	Our recommended dilutions for WB, IHC, and ELISA(peptide) are as follows: WB 1:1000-3000, IHC 1:50-1:200, and ELISA(peptide) 1:20000-1:40000. Please ensure that you use these dilutions to get the best possible results. By following these guidelines, you can ensure that your experiments are reproducible and that you get reliable and accurate data. If you have any questions or concerns about these recommended dilutions, do not hesitate to reach out to us for assistance. We are always happy to help you with your research needs and provide you with the support you need to succeed.
Immunogen	ROS（Phospho-Tyr2274），，。
MW	264KD
Properties
Concentration	1mg/ml
purification	Via sequential chromatography on phospho- and non-phospho-peptide affinity columns, the antibody was purified from rabbit serum, which had been previously purified. I can provide detailed information on the purification process used to obtain this highly specific antibody.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Orphan receptor tyrosine kinase (RTK) that plays a role in epithelial cell differentiation and regionalization of the proximal epididymal epithelium. May activate several downstream signaling pathways related to cell differentiation, proliferation, growth and survival including the PI3 kinase-mTOR signaling pathway. Mediates the phosphorylation of PTPN11, an activator of this pathway. May also phosphorylate and activate the transcription factor STAT3 to control anchorage-independent cell growth. Mediates the phosphorylation and the activation of VAV3, a guanine nucleotide exchange factor regulating cell morphology. May activate other downstream signaling proteins including AKT1, MAPK1, MAPK3, IRS1 and PLCG2.
Tissue specificity	Expressed in brain. Expression is increased in primary gliomas.
Posttranslational modification	Phosphorylated. Probably autophosphorylates. Phosphorylation at Tyr-2274 is required for the interaction with PTPN6 that mediates ROS1 dephosphorylation (By similarity). Phosphorylation at Tyr-2274 stimulates the kinase activity and the activation of the ERK1 signaling cascade (By similarity). Phosphorylation at Tyr-2274 and/or Tyr-2334 recruits PTPN11.
Celluar localization	Plasma Membrane;
UniPort	P08922