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Phospho-PRKCB1 (Thr500) Rabbit Polyclonal Antibody#abs140226

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Description

Catalog-specification	Delivery time	USD price
abs140226-100ug	1-2 Weeks	301.0
abs140226-50ug	1-2 Weeks	201.0

Overview
catalog	abs140226
Other names	： PKC betaPKC-B、PKC-beta、PKCB、Prkcb、PRKCB I、PRKCB II、PRKCB1、PRKCB2、C beta 1、C beta 2、C betaC beta。C beta，。、、。 C beta，C beta 1C beta 2。C beta 1C beta 2，。 C beta，。，C beta。C beta，。，C beta，。C beta，。
Source	Rabbit
Specificity	The PRKCB1 (Phospho-Thr500) Antibody is capable of identifying the levels of endogenous PRKCB1, but only when it is phosphorylated specifically at Thr500. This means that the antibody will not detect PRKCB1 that has been phosphorylated at other sites, or unphosphorylated PRKCB1. Any detection of PRKCB1 in the sample will be solely based on its phosphorylation at Thr500.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Chicken;Rabbit;Sheep;Dog;Bovine;Horse;
Antigen	PRKCB1
Application	This is the recommended range for dilution factors in various experimental techniques: for Western blotting (WB), it is typically recommended to dilute the antibody at a ratio of 1:1000 to 1:3000; for immunofluorescence (IF) or immunocytochemistry (ICC), the antibody is usually diluted at a ratio of 1:100 to 1:500; and for enzyme-linked immunosorbent assay (ELISA) with a peptide antigen, the recommended dilution range for the antibody is 1:20000 to 1:40000. It is important to note that these dilution factors may vary depending on the specific antibody and experimental conditions used.
Immunogen	PRKCB1（Phospho-Thr500）。
MW	77kDa
Properties
Concentration	1mg/ml
purification	The process involves purifying the antibody from rabbit serum by utilizing sequential chromatography on affinity columns specific to phospho- and non-phospho-peptides.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the quality of the product, it is recommended to keep it stored at a temperature of -20 °C for a duration of one year. It is advised to refrain from subjecting the product to multiple freeze/thaw cycles so as to avoid any unwanted alterations in its composition.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. May participate in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (By similarity). Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription.
Posttranslational modification	Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity. Phosphorylation at Tyr-662 by SYK induces binding with GRB2 and contributes to the activation of MAPK/ERK signaling cascade (By similarity).
Celluar localization	Cytosol;Extracellular region or secreted;Nucleus;Plasma Membrane;
UniPort	P05771