Phospho-NMDAR2B (Tyr1474) Rabbit Polyclonal Antibody#abs131050

abs131050-50ug

1-2 Weeks

201

abs131050-100ug

1-2 Weeks

301

Description

Ionotropic glutamate receptors known as N-methyl-D-aspartate (NMDA) receptors play a crucial role in synaptic transmission and memory formation. These receptors have been found to be involved in long-term potentiation, which is a phenomenon where the efficiency of synaptic transmission is enhanced through activity-dependent mechanisms. The activation of NMDA receptor channels is thought to be fundamental in the neuroplasticity and learning process of the brain.

Other names

The Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B, also known as NMDA R2B or NR2B, is critical for normal brain function. This protein is encoded by the GRIN2B gene and belongs to the NMDA receptor family. It is an ionotropic receptor that is selectively permeable to calcium and is activated by glutamate binding. The NMDA R2B subunit plays a key role in synaptic plasticity and learning and memory processes. Mutations in the GRIN2B gene are associated with several neurological disorders, including epilepsy and intellectual disability. Understanding the function and regulation of NMDA R2B may provide new therapeutic targets for treating these disorders.

Source

Rabbit

Specificity

The Phospho-NMDAR2B (Tyr1474) Antibody is designed to detect naturally occurring levels of NMDAR2B. It is capable of detecting this protein only when it has undergone a specific type of chemical modification known as phosphorylation at a specific location: Tyrosine 1474. This antibody is used in many research settings to help scientists better understand the complex workings of NMDAR2B and its role in cellular processes. By detecting this modified form of the protein, researchers can glean valuable insights into the ways that different proteins work and interact with each other within living cells.

Predictive reaction species

Rabbit;Pig;Dog;Bovine;Horse

Application

For Western blot, the recommended dilution range is from 1:500 to 1:2000. When it comes to Immunohistochemistry, the suggested dilution range falls between 1:50 and 1:200. For Immunofluorescence/Immunocytochemistry, the ideal dilution range is between 1:100 and 1:500. As for ELISA, the recommended dilution range for peptide is 1:20000 to 1:40000. To paraphrase, the appropriate dilution ranges for Western blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA(peptide) are from 1:500 to 1:2000, 1:50 to 1:200, 1:100 to 1:500, and 1:20000 to 1:40000 respectively.

A peptide synthesized from the region of human NMDAR2B encompassing the phosphorylation site at Tyrosine 1474 has been developed. It exhibits significant similarity to the original protein sequence, but its arrangement has been rearranged. The primary aim of this synthetic peptide is to target the specific phosphorylation event and its associated signaling processes. By focusing on this critical region, researchers can gain insights into the functional relevance of Tyrosine 1474 phosphorylation in the context of NMDAR2B. This synthetic peptide opens up opportunities for further investigation and understanding of the underlying molecular mechanisms involved in the regulation of NMDAR2B activity.

Properties

1mg/ml

Purification

The highly specific antibody was obtained by purifying rabbit serum through a rigorous process of chromatography using affinity columns for phospho- and non-phospho-peptides. This ensured that the antibody was of the highest quality, with a greater ability to specifically recognize and target its intended antigen. The purity of the serum was rigorously maintained throughout the process, resulting in an antibody that is well-suited for use in a wide range of research and diagnostic applications. Overall, this process allowed for the production of an antibody with unparalleled precision and specificity.

Clonality

Polyclonal Antibody

To keep it fresh for a year, it's best to store it at a temperature of minus 20 degrees Celsius. Make sure to prevent repeated freeze and thaw cycles to preserve its quality and effectiveness.

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

 NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death (By similarity).

Tissue specificity

Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.

Celluar localization

Plasma Membrane;

Q13224