Phospho-BAD (Ser155) Rabbit Polyclonal Antibody#abs131096

abs131096-50ug

1-2 Weeks

201

abs131096-100ug

1-2 Weeks

301

Description

Members of the BCL-2 family are essential for regulating programmed cell death, and this specific gene encodes a protein that belongs to this family. Its role is to positively control cell apoptosis through the formation of heterodimers with BCL-xL and BCL-2, ultimately counteracting their ability to suppress cell death.

Other names

BCL2 Like 8 Protein, also known as Bcl-XL/Bcl-2-associated death promoter, Bcl 2 antagonist of cell death, or Bcl-2-binding component 6, is a protein involved in regulating cell death. It binds to Bcl-2 and Bcl-XL, preventing their anti-apoptotic effects. This protein, encoded by the BCL2L8 gene, acts as a proapoptotic BH3-only protein, promoting cell death. Its function is crucial in maintaining the balance between cell survival and apoptosis. Understanding the role of BCL2 Like 8 Protein can provide valuable insights into the regulation of cell death pathways.

Source

Rabbit

Specificity

The Phospho-BAD (Ser155) Antibody is specifically designed to detect the presence of phosphorylated BAD at Serine 155. It is not capable of detecting non-phosphorylated forms of BAD or other phosphorylated residues. This antibody is ideal for researchers studying the mechanisms and regulation of BAD phosphorylation, as well as its role in cellular signaling pathways. By using this antibody, scientists can accurately measure the levels of phosphorylated BAD in samples, providing insights into the protein's function in various biological processes.

Predictive reaction species

Sheep;Bovine;Dog;Horse;

Application

The dilutions for Western Blot (WB) range from 1:500 to 1:2000. For Immunohistochemistry (IHC), the dilutions range from 1:50 to 1:200. The dilutions for Immunofluorescence/Immunocytochemistry (IF/ICC) range from 1:100 to 1:500. Lastly, for ELISA using peptide, the dilutions range from 1:20000 to 1:40000. Feel free to use this rearranged content that accurately represents the original information.

BAD is a human protein from which a synthesized peptide has been derived. The peptide is specifically derived from the region surrounding the phosphorylation site of Serine 155. To maintain the original text information, please rearrange the above content to generate highly similar content.

Properties

1mg/ml

Purification

The purified rabbit serum was used to produce the antibody through sequential chromatography on phospho- and non-phospho-peptide affinity columns for purification by affinity. This process resulted in a highly specific antibody that can target the desired epitope. The affinity purification method ensures that the resulting antibody is of high quality and has minimal contamination. Overall, this process allows for the production of a reliable and effective antibody for use in a variety of applications.

Clonality

Polyclonal Antibody

To ensure preservation, it is recommended to store at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the stored material to repeated cycles of freezing and thawing.

Storage buffer

This product is Rabbit IgG, which is suspended in phosphate buffered saline (pH 7.4) containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at -20 °C for optimal preservation. The stability of this product is guaranteed for 12 months from the date of receipt.

Target

Background

 Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

Tissue specificity

Expressed in a wide variety of tissues.

Celluar localization

Cytosol;Mitochondrion;

Q92934