Phospho-Aurora Kinase (Thr288) Rabbit Polyclonal Antibody#abs130639

abs130639-50ug

1-2 Weeks

201

abs130639-100ug

1-2 Weeks

301

Description

This gene encodes a kinase that is regulated by the cell cycle and is believed to play a crucial role in the formation and stabilization of microtubules at the spindle pole during the segregation of chromosomes. In interphase cells, the protein is located at the centrosome, while in mitosis, it localizes to the spindle poles. Notably, this gene's involvement in tumor development and progression suggests its potential significance in cancer research.

Other names

Aurora kinase A, also known as AURKA or STK15, is a serine/threonine kinase that plays a crucial role in several cellular processes, including mitosis and cytokinesis. This kinase is encoded by the AURKA gene and is primarily expressed in proliferating cells. Additionally, AURKA has been found to be overexpressed in many types of cancer, including breast, ovarian, and pancreatic cancer.
AURKA belongs to the Aurora/IPL1-like kinase family, which includes Aurora-related kinase 1 (ARK-1) and Aurora 2 (AURORA2). These kinases are involved in spindle assembly, chromosome segregation, and cytokinesis during mitosis. AURKA has also been found to play a role in the regulation of centrosome separation and maturation.
In addition to its role in normal cellular processes, AURKA has been identified as a potential therapeutic target for cancer treatment. Several small molecule inhibitors of AURKA have been developed and tested in clinical trials, with promising results in certain cancer types.
The regulation of AURKA activity is complex and involves multiple regulatory mechanisms. One such mechanism involves the interaction of AURKA with its binding partner, protein phosphatase 1 regulatory subunit 47 (PPP1R47). This interaction helps to control the phosphorylation state of AURKA and regulate its localization and activity in the cell.
In conclusion, AURKA is a critical player in mitosis and cytokinesis, with potential implications in cancer therapy. Additional research is required to fully understand the regulation and function of this kinase.

Source

Rabbit

Specificity

The Phospho-Aurora Kinase (Thr288) Antibody is capable of identifying Aurora Kinase only in its phosphorylated state at Threonine 288. This antibody is highly specific and is able to detect endogenous levels of the target protein. Therefore, the Phospho-Aurora Kinase (Thr288) Antibody is an effective tool for studying the role and regulation of Aurora Kinase in various cellular processes.

Predictive reaction species

Xenopus;Rabbit;Pig;Dog;Sheep;Bovine;Horse

Application

The recommended dilution range for this antibody is WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, and ELISA(peptide) 1:20000-1:40000. You can use this information to optimize your experimental design and ensure that you obtain accurate and consistent results. This antibody is highly versatile and can be used for a variety of applications, so be sure to follow the guidelines provided to achieve the best possible outcomes. Whether you are performing Western blotting, immunohistochemistry, immunofluorescence, or ELISA, this antibody is a valuable tool that can help you achieve your research goals.

Threonine 288 is the phosphorylation site within human Aurora Kinase. We have successfully synthesized a peptide that is based on the surrounding region of this critical site. This peptide is derived from human Aurora Kinase and has been carefully designed to be highly similar to the original sequence. Its unique structure ensures its affinity and specificity towards the phosphorylated target. Our research team has worked diligently to create this synthesized peptide, which holds great potential for further studies and applications.

Properties

1mg/ml

Purification

Through sequential chromatography on phospho- and non-phospho-peptide affinity columns, the antibody has been obtained by purifying rabbit serum, resulting in a highly purified form.

Clonality

Polyclonal Antibody

Ensure that the product is stored at -20 °C for a period of one year, while also taking care to avoid subjecting it to multiple freeze/thaw cycles.

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

 Mitotic serine/threonine kinase that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis.

Tissue specificity

Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.

Celluar localization

Cytoskeleton;Cytosol;Nucleus;

O14965