Phospho-4E-BP1 (Thr36) Rabbit Polyclonal Antibody#abs131056

abs131056-50ug

1-2 Weeks

201

abs131056-100ug

1-2 Weeks

301

Description

Phosphorylation of 4E-BP1 plays a crucial role in activating cap-dependent translation. Specifically, when 4E-BP1 is phosphorylated, it no longer binds to eIF4E, which allows for the assembly of eIF4E into the EIF4F complex. This disruption of binding enables cap-dependent translation to occur. On the other hand, when 4E-BP1 is not phosphorylated, it binds to eIF4E, preventing its incorporation into the EIF4F complex and inhibiting cap-dependent translation. Therefore, phosphorylation acts as a regulatory mechanism, controlling the activation of cap-dependent translation.

Other names

The Eukaryotic translation initiation factor 4E-binding protein 1, commonly known as 4E-BP1 or 4EBP1, also goes by other names such as BP 1, eIF4E binding protein 1, and PHAS-I. It is a vital protein that regulates the initiation of protein synthesis.
4EBP1 interacts with eukaryotic initiation factor 4E (eIF4E), a critical factor in the binding of mRNA to the ribosome during the initiation phase of protein synthesis. When phosphorylated, 4EBP1 binds to eIF4E, preventing the initiation of protein synthesis.
Insulin regulates the phosphorylation of 4EBP1 and the initiation of protein synthesis in response to nutrient levels and growth factors. Several kinases can phosphorylate 4EBP1, including mTOR, a serine/threonine kinase that plays a crucial role in the regulation of protein synthesis.
The importance of 4EBP1 is highlighted by its role in several physiological processes, including development and metabolism, and diseases such as cancer.

Source

Rabbit

Specificity

The Phospho-4E-BP1 (Thr36) Antibody is designed to target and detect the presence of 4E-BP1 protein, specifically when it is phosphorylated on the Threonine 36 residue. This antibody is highly specific and can accurately determine levels of phosphorylation of 4E-BP1 in endogenous samples. The antibody effectively recognizes and binds to the phosphorylated 4E-BP1 protein, indicating the activation of downstream signaling pathways and cellular processes that are crucial for maintaining cellular homeostasis. The precise detection of phosphorylation at Thr36 by this antibody can help improve research studies and investigations related to the regulation of protein synthesis, cellular metabolism, and other biological processes.

Predictive reaction species

Chicken;Rabbit;Pig;Dog;Sheep;Horse;Bovine

Application

The recommended dilution for using this antibody in western blot is 1:500-1:2000, while for immunohistochemistry it's 1:50-1:200. If using it for immunofluorescence or immunocytochemistry, the ideal dilution range is 1:100-1:500. For ELISA (using peptide), you should dilute the antibody in a range of 1:20000-1:40000. With these dilution recommendations, you can use the antibody effectively in your research with confidence.

Human 4E-BP1 has been utilized to synthesize a peptide that is specifically derived from the region surrounding Threonine 36, a crucial phosphorylation site. To ensure a highly similar content, the synthesized peptide is designed to closely mimic the original information encoded in the human 4E-BP1 protein sequence. This peptide holds great potential for further research and investigation in various biological and biochemical studies.

Properties

1mg/ml

Purification

The purified rabbit serum provided the antibody after undergoing affinity purification through sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Clonality

Polyclonal Antibody

To preserve its quality, the product must be stored at a temperature of -20 °C for a year. Avoid subjecting it to repeated cycles of freezing and thawing to prevent any damage.

Storage buffer

This product is Rabbit IgG, which is dissolved in phosphate-buffered saline (pH 7.4) containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at a temperature of -20 °C. It has a stable shelf life of 12 months from the date of receipt.

Target

Background

The activity of EIF4E, a translation initiation regulator, is tightly controlled by a repressor mechanism. In its hypophosphorylated form, it competes with EIF4G1/EIF4G3 and binds strongly to EIF4E, effectively repressing translation. On the other hand, the hyperphosphorylated form of EIF4E dissociates from it, allowing for the interaction between EIF4G1/EIF4G3 and EIF4E, which initiates translation. This repressor plays a crucial role in mediating the regulation of protein translation in response to various signals, such as hormones, growth factors, and stimuli that activate the MAP kinase and mTORC1 pathways. By modulating EIF4E activity, it ensures precise control over protein synthesis in different cellular contexts.

Celluar localization

Cytosol;Nucleus;

Q13541