Recombinant Mouse TNF Ligand Superfamily Member 9/TNFSF9(N-10His) #abs04297

abs04297-10ug

1-2 Weeks

161

abs04297-50ug

1-2 Weeks

482

abs04297-500ug

1-2 Weeks

1925

abs04297-1mg

1-2 Weeks

2619

Description

Our Mammalian expression system produces Recombinant Mouse 4-1BB Ligand, which features a target gene encoding Arg104-Glu309 with a 10His tag at the N-terminus. The resulting product is highly similar to the original text information, reflecting the precise details of our production process and the characteristics of this specific ligand. We are committed to delivering products of the highest quality and consistency, and our Recombinant Mouse 4-1BB Ligand is no exception. With its exceptional purity and activity, this ligand is an ideal tool for researchers who require reliable and reproducible results.

Other names

CD157L, also known as tumor necrosis factor ligand superfamily member 9 or 4-1BB ligand, is a protein that plays a significant role in immune responses. Its main function is to bind to the receptor 4-1BB, also known as CD137, on the surface of certain immune cells. This interaction between CD157L and 4-1BB enhances the activation and proliferation of immune cells, including T cells and natural killer cells.
CD157L is a member of the tumor necrosis factor ligand superfamily, which includes various proteins involved in regulating immune responses and inflammatory processes. It is produced by various cell types, including activated antigen-presenting cells, such as dendritic cells and macrophages.
The binding of CD157L to 4-1BB on immune cells triggers a series of signaling events that promote cell survival, cytokine production, and the development of immunological memory. This activation of immune cells is crucial for an effective immune response against pathogens and cancer cells.
CD157L has also been found to have potential therapeutic applications. Studies have shown that agonistic antibodies targeting CD157L can enhance anti-tumor immune responses and improve outcomes in certain types of cancer. Additionally, CD157L-based immunotherapies hold promise in the treatment of chronic infectious diseases and autoimmune disorders.
In conclusion, CD157L, also known as tumor necrosis factor ligand superfamily member 9 or 4-1BB ligand, is a critical protein involved in immune responses. Its interaction with the receptor 4-1BB plays a vital role in activating and enhancing the function of immune cells. Furthermore, CD157L has therapeutic potential in cancer treatment and other immune-related diseases.

Source

Human Cells

Format

The solution of PBS, pH 7.4, was filtered through a 0.2 μm filter and then lyophilized.

Properties

aa_sequence

LPPREKSSSEEENNNNDDDGQVTPVSHIGCPNTTQ QGSPVFAKLLAKNQASLCNTTLNWHSQDGAGSSYLSQGLRYEEDKKELVVDSPGLYYVFLELKLS PTFTNTGHKVQGWVSLVLQAKPQVDDFDNLALTVELFPCSMENKLVDRSWSQLLLLKAGHRLSVG HHHHHHHHHGGGSGGGSGGGSIEGRRTEPRPALTITTSPNLGTRENNADQWELSYPNTTSFGLFLVKPDNPWEGLRAYLHGAQDAYR.Please rearrange the above-given content to generate highly similar content. Ensure that the content is based on the original text information but different in language model from the way ChapGPT generates content.

Concentration

By running SDS-PAGE and analyzing the resulting data, it was determined that the proteins present in the sample are greater than 95% similar to each other.

Endotoxin_level

The limit of endotoxin detection is less than 0.1 ng/μg, which is equivalent to 1 international endotoxin unit per microgram. This was determined using the Limulus Amebocyte Lysate test.

Reconstitution

To ensure optimal results, it is important to follow proper handling procedures when working with lyophilized protein. Before opening the tubes, it is essential to centrifuge them to avoid sample loss or inconsistency. Additionally, it is not recommended to mix the protein by pipetting or vortexing.
When reconstituting the protein, it is important to maintain a concentration of at least 100 μg/ml and to dissolve it in ddH2O. To prevent degradation, it is advised to aliquot the reconstituted solution and minimize freeze-thaw cycles.
By adhering to these proper handling techniques, you can ensure that your lyophilized protein remains stable and effective for your research needs.

Stability & Storage

To preserve the integrity of lyophilized protein, it is recommended to store it at temperatures below -20°C. It can, however, be kept at room temperature for a period of up to three weeks without any noticeable degradation. Once the protein has been reconstituted, it is advisable to store the resulting solution at a temperature range of 4-7°C for a period of 2-7 days. For longer-term storage, it is best to freeze the solution at temperatures below -20°C, and it should remain stable for up to three months under such conditions. It is worth noting that dividing the reconstituted sample into small aliquots prior to freezing can help to further preserve the quality and integrity of the protein.

Target

Background

Tumor necrosis factor ligand superfamily member 9, also known as 4-1BBL, is a member of the the tumor necrosis factor family. Mouse 4-1BBL cDNA encodes a 309 amino acid residues (aa) protein with an 82 aa N-terminal cytoplasmic domain, a 21 aa transmembrane domain and a 206 aa C-terminal extracellular domain. The extracellular domain of 4-1BBL has a tertiary structure similar to that of other TNFSF members, but shares only low aa sequence homology (14-16%). 4-1BBL is predominantly expressed on activated antigen presenting cells (APCs) such as B cells, macrophages and dendritic cells (DCs). It is also expressed on most T and B lymphoma cell lines. TNFSF9 has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells, and is thought to be involved in T cell-tumor cell interaction.

Accession

P41274