Recombinant Human TRAIL R3/TNFRSF10C/CD263 (C-6His) #abs04242

abs04242-10ug

1-2 Weeks

62

abs04242-50ug

1-2 Weeks

184

abs04242-500ug

1-2 Weeks

1177

abs04242-1mg

1-2 Weeks

1601

Description

Our Mammalian expression system is responsible for producing Recombinant Human TRAIL receptor 3. The gene encoding Ala26-Ala221 is expressed, with a 6His tag located at the C-terminus. To maintain similarity, please rearrange the provided information and ensure that the generated content is based on the original text. However, refrain from using ChapGPT-style formatting and instead deliver the content in a completely different manner.

Other names

DcR1, also known as Tumor Necrosis Factor Receptor Superfamily Member 10C or TNFRSF10C, is a decoy receptor for TRAIL/Apo-2L. It is characterized as a TRAIL receptor without a death domain or an intracellular domain. Other names for DcR1 include Lymphocyte Inhibitor of TRAIL, TNF-Related Apoptosis-Inducing Ligand Receptor 3, TRAIL Receptor 3, TRAIL-R3, CD263, TRID, and LIT. Its role as an antagonist decoy receptor makes it an important player in regulating TRAIL-induced apoptosis pathways.

Source

Human Cells

Format

pH 7.2, 150mM NaCl, and 20mM PB were combined and filtered through a 0.2 μm filter. The resulting solution was then lyophilized to obtain a dried product.

Properties

aa_sequence

GHHHHHHHHHHHTTTVAAVPEPAAGATNMTAAAETTPGSPMTTAAAETTPGSPMTTAAAETTPGT MTSAAAETTPGSPMTAAAETTSPGSMATARQEEEAQEQQTQAPEHGHSKTGEFGDNNCCPSAPV CFTESPENCACAQTVPCHSGTPHRTNCENSQCYTVDDVCRDSMKHCTKHQDQTSCSNNHSEQRCS KEGMCRPPHMAGVEQSVNNRDWTCAGYCENSVQMEPANPCNAEPEEHSTSPNENHSSSKDHKSDQ
：，。。

Concentration

The determination of greater than 95% by reducing SDS-PAGE is a reliable method to establish the protein purity.

Endotoxin_level

As determined by the LAL test, the concentration of less than 0.1 ng/μg (1 IEU/μg) is considered to be an extremely low level. Let's rearrange the information and generate a unique version of the content.

Activity

TRAIL-mediated cytotoxicity inhibition was evaluated using L-929 mouse fibroblast cells treated with TRAIL. The observed effective dose (ED50) for this inhibitory effect was found to be less than 200 ng/ml.

Reconstitution

Centrifuging tubes before opening is essential. Avoid mixing using vortex or pipetting methods. It is not advisable to reconstitute to a concentration lower than 100 μg/ml. Use ddH2O to dissolve the lyophilized protein. To prevent freeze-thaw cycles, it is recommended to aliquot the reconstituted solution. It is important to note that the following content is generated based on the original text information but presented in a significantly different manner.

Stability & Storage

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target

Background

Tumor Necrosis Factor Receptor Superfamily Member 10C (TNFRSF10C) is a glycosyl-phosphatidylinositol-linked membrane protein which binds TRAIL with high affinity. TNFRSF10C has the TRAIL-binding extracellular cysteine-rich domains, lacks the intracellular signaling domain. As a result, binding of TRAIL to TRAIL R3 doesn’t transduce an apoptosis signal. The expression of TRAIL R3 gene has been shown to protect cells bearing TRAIL R1 and/or TRAIL R2 from TRAIL-induced apoptosis.

Accession

O14798