Recombinant Mouse OSM #abs00999

abs00999-2ug

1-2 Weeks

138

abs00999-10ug

1-2 Weeks

344

abs00999-1mg

1-2 Weeks

6742

Species

Murine

Source

Escherichia coli.

Format

The concentrated solution in 2 × PBS with a pH of 7.4 was filtered through a 0.2 μm filter and then lyophilized. The resulting product was then mixed with 5% trehalose before undergoing the freeze-drying process. This process ensures the stability of the product and allows for easy storage and transportation. The end product can be easily reconstituted by adding water, making it convenient for use in various applications.

Properties

aa_sequence

GFLWGYHRE CSNSSSQNRG LLSQSSSQLN LQNLNTLTGN ESLLEPYIRL QNLNTPDLRA CACTQHSVAF PSEDTLRQLS KPHFLSTVYT TDRVLYQLDA LQKFLKTPAF PKLDSARHNI LGIRNNVFCM ARLILLHSNE PTLPETQEPD GQSTTRAASS VFNKINGSCG RENVFREDDW GFTRSRVGSV

Concentration

> 96 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that the content of rMuOSM is below 1 EU/μg. Let me generate a rephrased version based on the original information. Keep in mind that the generated content will have a significantly different structure as compared to the original text.

Activity

Standard comparison reveals full biological activity. The ED is equivalent.₅₀The specific activity of a certain substance can be reliably determined through a cell proliferation assay utilizing murine NIH-3T3 cells. The results show that the concentration required for such activity is less than 1.0 ng/ml, indicating a highly potent substance. This finding implies that the substance is capable of inducing significant cellular growth and proliferation, with a specific activity of greater than 1.0 × 10. Such results are critical in determining the efficacy of any chemical compound meant for cellular applications.⁶ IU/mg.

Reconstitution

Prior to opening this vial, we advise briefly centrifuging it to settle the contents. Reconstitute in sterile distilled water or an aqueous buffer with 0.1% BSA to reach a 0.1-1.0 mg/ml concentration. Divide stock solutions into working aliquots and keep them at ≤ -20°C. Dilute further as necessary in appropriate buffered solutions.

Stability & Storage

To ensure the stability of this lyophilized preparation, it is recommended to store it at 2-8°C. However, for long term storage, it is best to keep it at -20°C and in a desiccated state. Once reconstituted, the preparation can be kept at 2-8°C for up to a week. For optimal stability, divide the reconstituted preparation into smaller working aliquots and store them at -20°C to -70°C. It is important to avoid freezing and thawing the preparation repeatedly in order to maintain its integrity.

Target

Accession

P53347

Gene IDs

18413

References

References

1. Tanaka M, Miyajima A. 2003. Rev Physiol Biochem Pharmacol. 149:39-52.

2. Mosley B, De Imus C, Friend D, et al. 1996. J Biol Chem. 271:32635-43.

3. Malik N, Kallestad JC, Gunderson NL, et al. 1989. Mol Cell Biol. 9:2847-53.

4. Brown TJ, Rowe JM, Liu JW, et al. 1991. J Immunol. 147:2175-80.