Recombinant Human TFF3 #abs01272

abs01272-5ug

1-2 Weeks

138

abs01272-20ug

1-2 Weeks

344

abs01272-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in PBS with a pH of 7.4 was filtered through a 0.2 μm filter and then lyophilized. Let's create a similar text by rearranging the provided information while ensuring its accuracy.

Properties

aa_sequence

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Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method has shown that Recombinant Human TFF3 has an EU/μg measurement of less than 1. To create similar content, the information can be rephrased to say: The level of Recombinant Human TFF3 has been tested using the LAL method, resulting in a measurement of less than 1 EU/μg.

Activity

The biological activity of the tested substance was found to be fully equivalent to that of the standard, indicating that it is highly effective. The ED value also confirmed this finding. Overall, the results suggest that this substance has the potential to be a viable alternative to the standard and may offer significant benefits.₅₀Based on the results of a chemotaxis bioassay using human MCF-7 cells, it has been established that the concentration required for activity is below 10 μg/ml, indicating a specific activity exceeding 100 IU/mg. Let's reconstruct the information in a different format:
The chemotaxis bioassay conducted on human MCF-7 cells demonstrated that the effective concentration needed for activity is less than 10 μg/ml. This finding suggests a significant specific activity of over 100 IU/mg.

Reconstitution

To ensure the contents are properly settled, we suggest that you briefly centrifuge the vial before opening it. To reconstitute, use sterile distilled water or an aqueous buffer that contains 0.1% BSA, and aim for a concentration of 0.1-1.0 mg/mL. Stock solutions should be separated into working aliquots and kept at a temperature that is not warmer than -20 °C. For further dilutions, use appropriate buffered solutions.

Stability & Storage

For optimal storage conditions of this lyophilized preparation, it is recommended to keep it at 2-8 °C. However, for long-term storage, it is advised to store it at -20 °C, preferably in a desiccated state. Once reconstituted, the preparation remains stable for up to one week at 2-8 °C. To ensure maximum stability, it is recommended to divide the reconstituted preparation into smaller aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the aliquots to repeated freeze/thaw cycles.

Target

Accession

Q07654

Gene IDs

7033

References

References

1. Seib T, Blin N, Hilgert K, et al. 1997. Genomics, 40: 200-2.

2. Kirikoshi HandKatoh M. 2002. Int J Oncol, 21: 655-9.

3. Lu R, Wang X, Chen JP, et al. 2010. Sichuan Da Xue Xue Bao Yi Xue Ban, 41: 114-7.

4. Jagla W, Wiede A, Dietzmann K, et al. 2000. FASEB J, 14: 1126-31.

5. Takano TandYamada H. 2009. Endocr J, 56: 9-16.