Recombinant Human Hepatoma-Derived Growth Factor/HDGF (C-6His) #abs04207

abs04207-10ug

1-2 Weeks

146

abs04207-50ug

1-2 Weeks

436

abs04207-500ug

1-2 Weeks

2353

abs04207-1mg

1-2 Weeks

3201

Description

The Recombinant Human Hepatoma-Derived Growth Factor is a product of our E.coli expression system. The gene that codes for Met1-Tyr100 is utilized, with a 6His tag located at the C-terminus. This ensures that the protein is easily identifiable and separable from other compounds. The production of this growth factor is crucial in the field of medicine, as it has been found to play a critical role in the growth and proliferation of cells. With its high similarity to the naturally occurring hepatoma-derived growth factor, it promises to be an effective therapeutic agent for various diseases.

Other names

One of the proteins known as Hepatoma-Derived Growth Factor (HDGF) is closely related to High Mobility Group Protein 1-Like 2 (HMG-1L2). HDGF and HMG-1L2 are essentially the same protein but referred to by different names. These proteins play vital roles in various cellular processes. The identification and characterization of HDGF and HMG-1L2 have led to significant advancements in understanding their functions and potential therapeutic applications. With further research, scientists hope to unveil additional insights into the mechanisms and significance of HDGF and HMG-1L2 in biological systems.

Source

Escherichia coli.

Format

pH 7.5, Lyophilized from a solution filtered through a 0.2 μm filter consisting of 20mM Tris, 1mM DTT, and 1mM EDTA.

Properties

aa_sequence

YGWDIAVERRVNVKGKTFNYAKHSGKEWHEDAVGEEKSLDFKLPGFDKFVSTTKNMEAFQWMDKPALPYPKAGKEQMRFYPANDGWWQFLACFFKMFFM.WEWPDDYKHDKMENLVKRKGENQGFAGSNKKPFHKRQEYHFPFTLENFHAAGDDNHDGQYKAG+.

Concentration

The SDS-PAGE reduction method showed a protein purity of over 95%. Can you please provide alternative phrasing for this sentence based on the original text information, without using the ChapGPT content generation approach?

Endotoxin_level

The LAL test revealed that the level of endotoxin in the sample was less than 0.1 ng/μg (1 IEU/μg). This information can be rearranged to create a content that closely mirrors the original text but in a slightly different format.

Reconstitution

Ensure proper handling of lyophilized protein by always centrifuging tubes before opening and avoiding mixing by vortex or pipetting. To maintain concentration, do not reconstitute below 100 μg/ml and dissolve in ddH2O. Prevent degradation by minimizing freeze-thaw cycles with proper aliquoting. These steps can help preserve the integrity of the reconstituted solution.

Stability & Storage

The stability and storage conditions of lyophilized protein should be carefully considered. It is recommended to store lyophilized protein at temperatures below -20°C to maintain its quality. However, it can also be stored at room temperature for a shorter period of time, around 3 weeks.
Once the lyophilized protein is reconstituted and turned into a solution, it requires specific storage conditions. The reconstituted protein solution should be stored at a temperature range of 4-7°C. Under these conditions, the solution can maintain its stability for a duration of 2-7 days.
To extend the shelf life of the reconstituted protein solution, it is advisable to prepare aliquots and store them at temperatures below -20°C. By doing so, the reconstituted samples can remain stable for up to 3 months.
In summary, proper storage and handling of lyophilized protein and reconstituted solutions are crucial to ensure their stability and longevity. Storing lyophilized protein at temperatures below -20°C and reconstituted solutions at 4-7°C or below -20°C for longer-term storage are recommended practices.

Target

Background

Hepatoma-Derived Growth Factor (HDGF) is a primary member of the HDGF family, characterized by its cytoplasmic localization and possession of a single PWWP domain. It is abundantly expressed in the nucleus and cytoplasm of smooth muscle and endothelial cells. By exhibiting proliferative, angiogenic, and neurotrophic activities, HDGF plays a critical role in various cellular processes. Initially identified as a secreted mitogen from human hepatoma cell line Huh-7, HDGF has a particular affinity for heparin and is highly expressed in tumor cells, where it fuels proliferation. Moreover, HDGF exerts mitogenic effects on fibroblasts and functions as a transcriptional repressor. Emerging evidence has firmly established the association between HDGF expression and tumorigenesis, indicating its pivotal role in cancer growth. Thus, HDGF represents a promising target for therapeutic interventions in cancer treatment.

Accession

P51858