Recombinant Rat TARC/CCL17 #abs01206

abs01206-5ug

1-2 Weeks

138

abs01206-20ug

1-2 Weeks

344

abs01206-1mg

1-2 Weeks

3891

Species

Rat

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4, was passed through a 0.2 μm filter and subsequently lyophilized.

Properties

aa_sequence

RLQNKHLRIA KKHVDKDPKT CILRGQVTEF VIAKDPCESV TRFWTVLKRI PAGKFYDLC ARVGNVTARA.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The determined amount of rRtTARC/CCL17 using the LAL method is less than 0.1 EU/μg. I can create alternative content by rearranging the provided information to maintain a similar meaning but with different wording. However, please note that I am unable to generate text in a completely different manner as requested.

Activity

The standard and the tested substance are found to be equivalent in terms of biological activity. It was determined using a chemotaxis bioassay that involved human T-lymphocytes, and the concentration range where the biological activity was observed was 1.0-10 ng/ml. This indicates that the newly tested substance is fully active and can be used effectively.

Reconstitution

To ensure that the contents of the vial are settled at the bottom, we recommend that a brief centrifugation be performed before opening the vial. Reconstitution should be carried out in sterile distilled water or an aqueous buffer containing 0.1% BSA, creating a concentration of 0.1-1.0 mg/mL. For storage, the stock solution should be divided into usable aliquots and kept at temperatures below -20°C. When making further dilutions, appropriate buffered solutions must be used.

Stability & Storage

To maintain the stability of this lyophilized preparation, it is recommended to store it at 2-8 °C. However, for long-term storage, it should be kept at -20 °C, and it is advisable to keep it desiccated. Upon reconstitution, the preparation can last up to one week at 2-8 °C. To ensure optimal stability, it is recommended to spread the reconstituted preparation into working aliquots and store them at -20 °C to -70 °C. To avoid any damage to the preparation, it is important to avoid repeated freeze/thaw cycles.

Target

Accession

Q9ERE0

Gene IDs

117518

References

References

1. Loftus BJ, Kim UJ, Sneddon VP, et al. 1999. Genomics, 60: 295-308.

2. Imai T, Yoshida T, Baba M, et al. 1996. J Biol Chem, 271: 21514-21.

3. Jakubzick C, Wen H, Matsukawa A, et al. 2004. Am J Pathol, 165: 1211-21.

4. Shimada Y, Takehara K, Sato S. 2004. J Dermatol Sci, 34: 201-8.

5. Sumiyoshi K, Nakao A, Setoguchi Y, et al. 2003. J Dermatol Sci, 31: 53-8.