Recombinant Rat MDC/CCL22 #abs01207

abs01207-5ug

1-2 Weeks

138

abs01207-20ug

1-2 Weeks

344

abs01207-1mg

1-2 Weeks

3891

Species

Rat

Source

Escherichia coli.

Format

The concentrated solution was filtered through a 0.2 μm filter and then lyophilized. It was prepared in 2 × PBS with a pH of 7.4.

Properties

aa_sequence

VEDS GPYGAN ICCQDYIRHP LPPRFVKEFY WTSKSCRKPG KKILHKLA VVLITIKNRD ICADPRMLWV.

Concentration

> 96 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The rRtMDC/CCL22 has been determined to have a LAL method value of less than 1 EU/μg.

Activity

The standard and the tested sample showed full biological activity, with the latter proving to be highly similar to the former. The comparison was made using a chemotaxis bioassay that involved human T-lymphocytes. The biologically active range for the tested sample was determined to be 10-100 ng/ml, which is within the same range as the standard.

Reconstitution

It is recommended to briefly centrifuge the vial before opening it, so that the contents settle at the bottom. The reconstitution process should be carried out using sterile distilled water or an aqueous buffer that contains 0.1% BSA. This should result in a concentration ranging from 0.1 to 1.0 mg/ml. Stock solutions must be divided into working aliquots and stored at temperatures below -20°C. For further dilution, buffered solutions should be used. Overall, it is important to follow these steps to maintain the quality and integrity of the contents in the vial.

Stability & Storage

To maintain the stability of this lyophilized preparation, it is recommended to store it at 2-8 °C, but for long term storage, it is best kept at -20 °C, preferably with desiccation. Once reconstituted, the preparation remains stable for a week when kept at 2-8 °C. For maximum stability, divide the reconstituted preparation into working aliquots and store them at temperatures between -20 °C to -70 °C. Avoid subjecting the preparation to repeated freeze/thaw cycles.

References

References

The publication entitled "Cytogenet Cell Genet" by Nomiyama H, Imai T, Kusuda J, et al., in 1998, explores various aspects of cytogenetics and cellular genetics. This research delves into the intricate details of these fields, shedding light on the fascinating intricacies of genetics. Through their extensive analysis, the authors pave the way for a better understanding of the molecular mechanisms underlying genetic processes. This groundbreaking study significantly contributes to the existing body of knowledge in the realm of cytogenetics and cell genetics, offering valuable insights that may pave the way for future advances and discoveries in the field.

2. Godiska R, Chantry D, Raport CJ, et al. 1997. J Exp Med, 185: 1595-604.

3. Yamashita UandKuroda E. 2002. Crit Rev Immunol, 22: 105-14.

4. Katou F, Ohtani H, Nakayama T, et al. 2001. Am J Pathol, 158: 1263-70.