Recombinant Human Eotaxin-2/CCL24 #abs01143

abs01143-5ug

1-2 Weeks

138

abs01143-20ug

1-2 Weeks

344

abs01143-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl was filtered through a 0.2 μm filter and then lyophilized.

Properties

aa_sequence

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Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The Recombinant HumanEotaxin-2/CCL24 has been determined to have less than 1 EU/μg by the LAL method. This ensures that the product is of high quality and free from endotoxins. Follow proper storage and handling protocols to maintain the purity of the product. It is important to take note of the recommended dosage and usage instructions to prevent adverse effects.

Activity

The standard and the fully biologically active form show comparable levels. The concentration required to observe this biological activity was determined through a chemotaxis bioassay utilizing eosinophils from human peripheral blood. Specifically, a concentration range of 50-100 ng/ml was found to induce the desired response.

Reconstitution

We suggest a brief centrifugation of the vial prior to opening to settle the contents at the bottom. For reconstitution, use sterile distilled water or an aqueous buffer with 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Stock solutions need to be divided into working aliquots and stored at ≤ -20 °C. Any further dilutions should be made using appropriate buffered solutions. It is crucial to follow these procedures for optimal results.

Stability & Storage

To ensure the stability of this lyophilized preparation, it should be stored at a temperature range of 2-8 °C. However, for long-term storage, it is recommended to keep the preparation at a temperature of -20 °C, while also keeping it desiccated. Upon reconstitution, the preparation can remain stable for up to a week, if stored at a temperature range of 2-8 °C. For optimal stability, it is suggested that you apportion the reconstituted preparation into working aliquots and keep them stored at a temperature range of -20 °C to -70 °C. Avoid multiple freeze/thaw cycles, as this could damage the preparation.

Target

Accession

O00175

Gene IDs

6369

References

References

1. Owczarek W, Paplinska M, Targowski T, et al. 2010. Cytokine, 50: 181-5.

2. Ben-Yehuda C, Bader R, Puxeddu I, et al. 2008. Exp Lung Res, 34: 467-79.

3. Schaefer D, Meyer JE, Pods R, et al. 2006. Int Arch Allergy Immunol, 140: 205-14.