Recombinant Rat NAP-2/CXCL7 #abs01193

abs01193-2ug

1-2 Weeks

138

abs01193-10ug

1-2 Weeks

344

abs01193-1mg

1-2 Weeks

3891

Species

Rat

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized. A highly similar statement can be generated as follows: Following concentration in PBS, pH 7.4, the solution was filtered through a 0.2 μm filter and then subjected to lyophilization.

Properties

aa_sequence

The content generated by rearranging the given text is as follows:
VIGNRPPGAW DTEAKIVCNL KIKVMRCNVT TIIALNIVCE PNIICRVCLR RLVCPPGEF KKPITVVKMN.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that rRtNAP-2/CXCL7 has less than 1 EU/μg. It is important to note that this content should be highly similar to the original text, but rearranged in a different way for clarity.

Activity

The standard and the sample were compared, and it was found that the sample is fully biologically active. This was determined by conducting a chemotaxis bioassay using human CXCR2 transfected murine BaF3 cells. The concentration range determined for this biological activity is between 0.1-1.0 ng/ml. It can be concluded that the sample is highly similar to the standard in terms of its biological activity.

Reconstitution

We suggest a brief centrifugation of the vial before opening to settle the contents at the bottom. To create a solution, reconstitute the contents in sterile distilled water or an aqueous buffer with 0.1% BSA to achieve a concentration of 0.1-1.0 mg/ml. Store the stock solutions in working aliquots at ≤ -20 °C. Subsequent dilutions should be performed in appropriate buffered solutions.

Stability & Storage

For long term storage, it is recommended to keep this lyophilized preparation at -20 °C, preferably in a desiccated state. However, it can also maintain stability at 2-8 °C. Once reconstituted, it remains stable for up to one week when stored at 2-8 °C. To ensure maximum stability, it is advised to divide the reconstituted preparation into separate working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles.

References

References

1. Hristov M, Zernecke A, Bidzhekov K, et al. 2007. Circ Res. 100:590-7.

2. Majumdar S, Gonder D, Koutsis B, et al. 1991. J Biol Chem. 266:5785-9.

3. Krijgsveld J, Zaat SA, Meeldijk J, et al. 2000. J Biol Chem. 275:20374-81.

4. Piccardoni P, Evangelista V, Piccoli A, et al. 1996. Thromb Haemost. 76:780-5.