Recombinant Rat CINC-2α/CXCL3 #abs01190

abs01190-2ug

1-2 Weeks

138

abs01190-10ug

1-2 Weeks

344

abs01190-1mg

1-2 Weeks

3891

Species

Rat

Source

Escherichia coli.

Format

After concentration, a solution in 20 mM PB with a pH of 7.4 and 50 mM NaCl was filtered through a 0.2 μm filter and lyophilized. A highly similar content can be created by rearranging the information provided, based on the original text.

Properties

aa_sequence

KSSSDKLLKQ IIQKILQRPL PAQCNLCEVQ DGEKDTLKAT LIVEVTQTCT HPGPTPVTLS QINEFDVRLP TKCLQCRLEL
Rephrased:
The sequence of letters RELRCQCLKT LPRVDFENIQ SLTVTPPGPH CTQTEVIATL KDGQEVCLNP QAPRLQKIIQ KLLKSDKSS can be rearranged to read KSSSDKLLKQ IIQKILQRPL PAQCNLCEVQ DGEKDTLKAT LIVEVTQTCT HPGPTPVTLS QINEFDVRLP TKCLQCRLEL. This new arrangement retains the information in the original text but presents it in a different pattern of letters.

Concentration

> 96 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The rRtCINC-2α/CXCL3 has been tested and found to contain less than 1 EU/μg using the LAL method. It is important to ensure that the content generated is similar to the original text information.

Activity

The standard and the fully biologically active form show comparable levels of activity. In order to determine the biological activity, a chemotaxis bioassay was performed using human CXCR2 transfected murine BaF3 cells. The concentration range for this activity was found to be between 5-50 ng/ml.

Reconstitution

Before opening, it is recommended to briefly centrifuge the vial in order to settle the contents at the bottom. To achieve a concentration of 0.1-1.0 mg/mL, reconstitute the vial in sterile distilled water or a aqueous buffer containing 0.1 % BSA. It is advisable to divide the stock solutions into working aliquots and store them at a temperature of ≤ -20 °C. For further dilutions, appropriate buffered solutions should be used.

Stability & Storage

The lyophilized form of this product can be kept in a cool environment of 2-8 °C, but for long-term storage, it's recommended to store it at a temperature of -20 °C after the desiccation process. Once reconstituted, the product can last up to a week at 2-8 °C. It's advisable to divide the reconstituted preparation into smaller quantities and store them within working aliquots at a temperature range of -20 °C to -70 °C for optimal stability. Avoid using the product after several freeze/thaw cycles.

Target

Accession

Q10746

Gene IDs

171551

References

References

1. Nolan KF, Strong V, Soler D, et al. 2004. J Immunol. 172:2201-9.

2. Modi WS, Yoshimura T. 1999. Mol Biol Evol. 16:180-93.

3. Tsai HH, Frost E, To V, et al. 2002. Cell. 110:373-83.

4. Smith DF, Galkina E, Ley K, et al. 2005. Am J Physiol Heart Circ Physiol. 289:H1976-84.

5. Nakagawa H, Komorita N, Shibata F, et al. 1994. Biochem J. 301 ( Pt 2):545-50.