Recombinant Mouse MEC/CCL28 #abs01186

abs01186-5ug

1-2 Weeks

138

abs01186-20ug

1-2 Weeks

344

abs01186-1mg

1-2 Weeks

3891

Species

Murine

Source

Escherichia coli.

Format

The concentrated solution in 20 mM PB with a pH of 7.4 and containing 150 mM NaCl has been subjected to lyophilization after passing through a 0.2 μm filter. A similar content can be created by rearranging this information to preserve the original meaning.

Properties

aa_sequence

SDCCHKHGHI SSRHKHEHAT TRRKTTNRGA LKHHGTHRWE CSCIHAEGVD AGQDLDIIRS KVESINTLSR RMGSRCASPV KRTQKRKSNH NKVMKREQGC VCAVRKNRTK RSRDTHETHT.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that the rMuMEC/CCL28 has an EU/μg value below 1. Here is an alternative way to express the same information: The LAL method was used to calculate the concentration of rMuMEC/CCL28, and the result showed that it is less than 1 EU/μg.

Activity

The standard and the fully biologically active substance have comparable activity levels. Murine lymphocytes were used to determine their biological activity through a chemotaxis bioassay, with a concentration range of 1.0-10.0 ng/ml.

Reconstitution

Before opening this vial, it is advisable to briefly centrifuge it to settle the contents at the bottom. Reconstitute the contents in sterile distilled water or an aqueous buffer that includes 0.1% BSA, aiming for a concentration range of 0.1-1.0 mg/mL. Divide the resulting stock solution into smaller working aliquots and store them at a temperature not exceeding -20 °C. For further dilutions, please use suitable buffered solutions.

Stability & Storage

To ensure the stability of this lyophilized preparation, it is recommended to store it at 2-8 °C. However, for long-term storage, it is best to keep it at -20 °C, ideally desiccated. Once reconstituted, the preparation can remain stable for up to a week if stored at 2-8 °C. For optimal stability, it is recommended to divide the reconstituted preparation into small working aliquots and store them at temperatures between -20 °C and -70 °C. To avoid any potential damage to the preparation, it is important to avoid repeated freeze/thaw cycles.

Target

Accession

Q9JIL2

Gene IDs

56838

References

References

1. Wang W, Soto H, Oldham ER, et al. 2000. J Biol Chem, 275: 22313-23.

2. Hieshima K, Ohtani H, Shibano M, et al. 2003. J Immunol, 170: 1452-61.

3. Eksteen B, Miles A, Curbishley SM, et al. 2006. J Immunol, 177: 593-603.

4. Kagami S, Kakinuma T, Saeki H, et al. 2005. J Invest Dermatol, 124: 1088-90.