Recombinant Mouse MIP-3β/CCL19 #abs01179

abs01179-5ug

1-2 Weeks

138

abs01179-20ug

1-2 Weeks

344

abs01179-1mg

1-2 Weeks

3891

Species

Murine

Source

Escherichia coli.

Format

The concentrated solution in PBS, with a pH of 7.4, was filtered through a 0.2 μm filter and then lyophilized. To produce content that closely mirrors the original text, the above sentence can be rearranged and rephrased as follows: After concentration, the solution was treated with a 0.2 μm filter and then subjected to lyophilization. The pH of the solution was 7.4, and it was stored in PBS throughout the process.

Properties

aa_sequence

QVPSKSNNRG KSSAAPPDPV RRSTRRSKNK GNSTRRSKNK SIGVAVVCEC GPATVPRQSD TDDQPPOKIRT YCFTQKRLGN LLYANFVRKI CLSVEGDPRL.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method indicates that the rMuMIP-3β/CCL19 has less than 1 EU/μg. Can we generate some content with the same meaning by rearranging the original text?

Activity

The biological activity of the substance under examination has been found to be fully comparable with the standard. A chemotaxis bioassay conducted using human mature dendritic cells has determined that its concentration range of 10-100 ng/ml exhibits biological activity.

Reconstitution

To ensure the potency of the vial's contents, we recommend a brief centrifugation prior to opening, which will bring everything to the bottom. From there, reconstitute in sterile distilled water or an aqueous buffer that includes 0.1% BSA. Aim for a concentration of 0.1-1.0 mg/mL. It's best to separate stock solutions into working aliquots, and store them at or below -20°C. For further dilutions, select appropriate buffered solutions.

Stability & Storage

To ensure the stability of this lyophilized preparation, it is recommended to keep it at 2-8 °C. However, for long term storage, it is best to store it at -20 °C, preferably in a desiccated state. When reconstituted, the preparation can be kept stable for up to one week when stored at 2-8 °C. To maintain its stability for a longer period of time, it is advisable to divide the reconstituted preparation into working aliquots and keep them stored at -20 °C to -70 °C. It is important to avoid exposing the preparation to repeated freeze/thaw cycles. Following these guidelines will help preserve the quality of this preparation and ensure its effectiveness in use.

Target

Accession

O70460

Gene IDs

24047

References

References

1. Milicevic NM, Miljkovic MD, Milicevic Z, et al. 2011. Histochem Cell Biol, 135: 593-601.

2. Guerin LR, Moldenhauer LM, Prins JR, et al. 2011. Biol Reprod, 85: 397-408.

3. Yoshida R, Imai T, Hieshima K, et al. 1997. J Biol Chem, 272: 13803-9.

4. Gibejova A, Mrazek F, Subrtova D, et al. 2003. Am J Respir Crit Care Med, 167: 1695-703.