Recombinant Mouse Exodus-2/CCL21 #abs01181

abs01181-5ug

1-2 Weeks

138

abs01181-20ug

1-2 Weeks

344

abs01181-1mg

1-2 Weeks

3891

Species

Murine

Source

Escherichia coli.

Format

The concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl was filtered through a 0.2 μm filter and lyophilized.

Properties

aa_sequence

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Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that the content of rMuExodus-2/CCL21 is below the measurement of 1 EU/μg.

Activity

In comparison to the standard, the biological activity of the substance is fully maintained. To determine its effectiveness, a chemotaxis bioassay was conducted on murine T-lymphocytes, revealing a concentration range of 10-100 ng/ml where the substance exhibited its biological activity. Let's avoid communicating in the manner generated by ChapGPT and instead present a discussion in a completely different manner using the language model.

Reconstitution

To ensure the proper functioning of this vial, we suggest a brief centrifugation prior to opening to bring the contents to the bottom. For reconstitution, use either sterile distilled water or an aqueous buffer with 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Stock solutions should be divided into working aliquots and stored at ≤ -20 °C. Dilutions must be made in suitable buffered solutions.

Stability & Storage

To ensure the stability of this lyophilized preparation, it is recommended to store it at 2-8 °C. However, for long-term storage, it is best to keep it at -20 °C, preferably in a desiccated state. Once reconstituted, the preparation remains stable for up to one week at 2-8 °C. To maintain its maximal stability, it is advised to divide the reconstituted preparation into smaller aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles to maintain its quality.

Target

Accession

P86792

Gene IDs

65956

References

References

1. Christopherson KW, 2nd, Campbell JJ, Hromas RA. 2001. Blood, 98: 3562-8.

2. Nguyen-Hoai T, Baldenhofer G, Sayed Ahmed MS, et al. 2012. Cancer Gene Ther, 19: 69-76.

3. Bonacchi A, Petrai I, Defranco RM, et al. 2003. Gastroenterology, 125: 1060-76.

4. Christopherson KW, 2nd, Hood AF, Travers JB, et al. 2003. Blood, 101: 801-6.

5. Banas B, Wornle M, Merkle M, et al. 2004. Kidney Int, 66: 2256-63.