Recombinant Human MEC/CCL28 #abs01147

abs01147-5ug

1-2 Weeks

138

abs01147-20ug

1-2 Weeks

344

abs01147-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl was filtered through a 0.2 μm filter and then lyophilized.

Properties

aa_sequence

QNHHTPKGTG KHEGAHQRNS SRNKGRHHKK HVCLSVCRIR AKVMWQKQVT HNHPVSCIRV RHVKLIVALA CDGCDAGRRI CMNVRELRLR SHHVSEVTCG CEOHOINGGK GKNGGKNKKA AAVQKVMQWT PHGRPAPGDR.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method has indicated that Recombinant HumanMEC/CCL28 has less than 1 EU/μg. It is important to note that this determination reflects a high degree of similarity to the original text information.

Activity

The standard and the fully biologically active substance have been compared, and it has been determined that the biological activity of the substance is similar to the standard. This determination was made using a chemotaxis bioassay that utilized human lymphocytes. The concentration range in which the substance displayed its biological activity was found to be between 1.0-10.0 ng/ml.

Reconstitution

To ensure optimal results, it is recommended to briefly centrifuge the vial before opening to bring the contents to the bottom. Reconstitution should be carried out using sterile distilled water or an aqueous buffer that contains 0.1 % BSA, in order to achieve a concentration of 0.1-1.0 mg/mL. Stock solutions need to be divided into working aliquots and stored at or below -20 °C. Dilutions should be made using appropriate buffered solutions.

Stability & Storage

To maintain the stability of this lyophilized preparation, it is recommended to store it at 2-8 °C, although for long-term storage, keeping it at -20 °C is preferable. Desiccation should also be considered. Once reconstituted, the stability of the preparation is guaranteed for up to one week if kept at 2-8 °C. However, for optimal stability, any remaining preparation should be separated into workable portions and stored at -20 °C to -70 °C. Repeated freeze/thaw cycles should be avoided.

Target

Accession

Q9NRJ3

Gene IDs

56477

References

References

1. Wang W, Soto H, Oldham ER, et al. 2000. J Biol Chem, 275: 22313-23.

2. Hieshima K, Ohtani H, Shibano M, et al. 2003. J Immunol, 170: 1452-61.

3. Eksteen B, Miles A, Curbishley SM, et al. 2006. J Immunol, 177: 593-603.

4. Kagami S, Kakinuma T, Saeki H, et al. 2005. J Invest Dermatol, 124: 1088-90.