Western Primary Antibody Secondary Antibody Removing Solution(Acidic)

1. Start by removing the exposed membrane and then add an appropriate amount of regeneration solution. Make sure the regeneration solution fully covers the membrane's surface. For an 8.5cm × 5.5cm membrane, around 15 mL of regeneration solution should suffice. Shake the membrane gently and incubate it at room temperature for approximately 15 minutes. However, the incubation time can vary depending on the target proteins. If you are dealing with proteins that have a high expression level, such as internal reference antibodies, you may want to extend the incubation time to 1 hour or 30 minutes at 37℃ when using the regeneration solution.
2. Once the incubation is complete, discard the regeneration solution and proceed to wash the membrane with 15 mL of buffer solution (PBST or TBST). Perform this washing step three times for 5 minutes each time. Shake the membrane gently during the washing process and maintain room temperature.
3. To ensure complete elution of the hrP-conjugate secondary antibody, you can utilize a color method to confirm its elution. This step helps in determining whether the secondary antibody has been fully removed from the membrane.
4. After the testing is finished, verify that there is no remaining enzyme activity on the membrane. Once confirmed, seal the membrane by adding 15 mL of sealing solution and allow it to seal at room temperature for 30 minutes. Alternatively, you can seal it overnight in the refrigerator at 4℃.
5. Finally, reintroduce the primary antibody you wish to test and proceed with the next round of WB experiments.

1, mild formula, does not contain mercaptoethanol and other reagents; 2, suitable for the use of NC or PVDF membrane and other transfer film.

Store at 2-8℃ for 12 months

Western blotting membrane regeneration solution (acid); Western Blot Antibody Stripping Solution

pH=2.0