Drift Antigen Retrieval Solution(10×)

Please note that the price mentioned above is only for your reference. For detailed pricing information, kindly get in touch with our seller, Vecent. Feel free to reach out to Vecent for further assistance.

Send Inquiry

Description

SKU-Pack Size	Availability	Price
abs9209-100ml	In stock	$30.00

Description
Description	When cells or tissues are treated with paraformaldehyde, formaldehyde, or other aldehyde reagents for fixation, it leads to the formation of cross-links between proteins. These cross-links often mask the antigen sites present on the samples, resulting in a reduced staining signal during immunostaining. In some cases, false positive staining may also occur as a consequence. It is important to be aware of this effect as it can impact the accuracy and reliability of immunostaining results. The Floating (10× Sections) Antigen Retrieval Solution is formulated with sodium citrate and other antigen repair reagents, resulting in a pH value of approximately 8.5. This solution effectively minimizes the risk of drift fragmentation and eliminates protein cross-linking caused by aldehyde fixation reagents. By fully exposing the epitopes in paraffin sections and various samples, it significantly enhances the quality of immunostaining. This versatile solution is suitable for repairing antigens in drift plates and can be applied for antigen retrieval in paraffin sections, frozen sections, and other sample types. Paraffin sections typically require antigenic repair, whereas frozen sections do not necessitate it. Repairing the antigens can significantly enhance immunostaining in paraffin sections, but there is also substantial literature demonstrating notable improvements in frozen sections. When immunostaining on frozen sections proves ineffective, one may consider trying antigenic repair. Regardless of whether it is a frozen section or a cell slide, as long as paraformaldehyde, formaldehyde, or other aldehyde reagents are employed for fixation, antigen repair can effectively dismantle the protein cross-linking, fully exposing the antigen epitopes, and greatly enhancing the immunostaining outcome.
Usage	1. Paraffin section: < br > The following steps were followed during the preparation of paraffin sections. First, the sections were dewaxed in xylene for two rounds of 5 minutes each. They were then transferred to anhydrous ethanol for two rounds of 5 minutes each. Subsequently, the sections were treated with 90% ethanol for two rounds of 5 minutes each and then with 70% ethanol for 5 minutes. Finally, the sections were washed with distilled water in two rounds of 5 minutes each. To perform antigen repair on the sections, the first step is to soak them in antigen repair solution. It is recommended to heat the sections at 80℃ for around 30 minutes. However, the optimal heating time might differ for different samples and target proteins. It is crucial to preheat the antigen repair solution (1×) to 80℃ before use, which can be done in a water bath. Thereafter, the sections should be cooled down to room temperature for about 20 to 30 minutes. To prepare for subsequent immunostaining steps, wash the sections with immunostaining solution one to two times, with each wash taking 3 to 5 minutes. Once the sections are rinsed properly, blocking can be carried out. Accuracy, precision, and care need to be taken in each step of the protocol to ensure high-quality results. 2. Frozen sections: the sections were washed with immunostaining solution for 5min. The process of antigenic repair as in paraffin sectioning. 3. For other samples, the antigenic repair can be performed by referring to paraffin section or frozen section.
Storage Temp.	2 ~ 8 ℃. If not used for a long time, please store at -20℃.
Properties
Synonym	Antigen-repair solution for bleach

Hot Tags: drift antigen retrieval solution(10×), China drift antigen retrieval solution(10×) suppliers

Previous:DAB Chromogenic Kit(brown Yellow, IHC)

Next:Cell Climbing Fragment Antigen Retrieval Solution(1X)

Send Inquiry