DAB Chromogenic Kit(brown Yellow, IHC)
Please note that the mentioned price is provided for your reference only. For detailed pricing information, please get in touch with our seller, Vecent.
Description
| SKU-Pack Size | Availability | Price |
| abs9210-1kit | In stock | $90.00 |
Please note that the mentioned price is provided for your reference only. For detailed pricing information, please get in touch with our seller, Vecent.
Description | |
| Description | 3,3'-diaminobenzidine (DAB), also known as DAB, is an essential substrate of peroxidase. When catalyzed, it produces brown-yellow products that are insoluble in water. These products can be easily seen with the naked eye, making DAB suitable for various staining procedures in spot detection and immunohistochemistry. Rearranging the content based on the original text reveals the significance of DAB as a chromogenic substrate. By undergoing enzymatic reactions, DAB forms visible brown-yellow compounds, crucial for staining and detecting spots in immunohistochemistry applications. Its water-insoluble nature allows easy visual observation during staining procedures. In summary, DAB plays a vital role in various steps of spot detection and immunohistochemistry, providing a simple and effective means for visualizing peroxidase activity. |
| Usage | 1. Following incubation in the peroxidase detection system, fully wash with TBS or PBS for a duration of 5 minutes, repeating the washing process 4 times. 2. Prepare a solution by adding 1 drop each of substances A, B, and C to every milliliter of distilled water. Thoroughly mix the solution and add it to the specimen. The color development typically takes 1-10 minutes, and it should be observed under a microscope. If no background is observed, the color development can proceed. In case the color development is too rapid, consider reducing the concentration of the DAB staining by diluting 1 drop of substances A, B, and C in 1.5ml or 2ml of distilled water. Conversely, if the color development is too slow, it may be increased by adding 3 drops of color solution A, B, and C to each 2ml of distilled water. 3. To stop the reaction, thoroughly wash the sample with distilled water. If necessary, apply a restaining process using Mayor's hematoxylin for a duration of 1-2 minutes, followed by water washing. 4. Dehydrate the specimen, apply a transparent and neutral gum seal, and observe under an optical microscope. |
Overview | |
| Storage Temp. | Stored at -20℃ for 1 year |
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