Rat IFN-γ ELISA KIT

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Testing Principle

In this experiment, we employed the double antibody sandwich ELISA technique. Initially, a microplate was coated with an anti-rat IFN-γ monoclonal antibody. This antibody acted as a solid support for capturing IFN-γ present in both the sample and standard. Subsequently, any unbound components were eliminated through a careful washing step. To detect the captured IFN-γ, a biotin-labeled anti-rat IFN-γ polyclonal antibody was introduced, allowing it to specifically bind to the IFN-γ already attached to the microplate. Again, any unbound substances were washed away. To further amplify the signal, streptavidin-labeled horseradish peroxidase was employed, specifically recognizing and binding the biotin present on the polyclonal antibody. After a final washing step, a chromogenic substrate solution was added, resulting in a gradual color change of the solution towards blue. To halt this reaction and fix the color, a stop solution was introduced, turning the solution yellow. Finally, the absorbance of the solution was quantified using a microplate analyzer. This experimental procedure allowed for the accurate measurement of IFN-γ levels in the samples.

Detection type: sandwich enzyme immunoassay

Form:pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity: 100ul

Kit composition:

The package includes a standard 96-well plate that is pre-packed with all the necessary components for anti-rat IFN-γ detection. It also comes with a dilution buffer and chromographic solutions A and B, as well as a washing solution, stop solution, and SA-HRP. Additionally, the package contains a sealing plate membrane and an instruction manual to guide you through the process.

Sensitivity: 15.6 pg/mL

Detection range: 39.1 - 2,500 pg/mL

Recovery rate:89.0-118.9%

Storage: 2-8℃

The standard curve:

Background:

IFN-γ is a pro-inflammatory cytokine that is typically produced by various immune cells when the body is under inflammatory conditions. It is predominantly produced by T cells and NK cells. The primary function of IFN-γ is to aid in host defense through promotion of Th1 cell development and activation, as well as by chemically attracting and activating monocytes and macrophages. It also induces upregulation of antigen-presenting molecules and immunoglobulin class conversion in B cells, in addition to displaying both antiviral and anti-proliferation properties.
IFN-γ is a dimer that signals through receptor complexes comprising two IFN-γR1 and two IFN-γR2 subunits. Moreover, it has exhibited anti-inflammatory properties by promoting the development of regulatory T cells while inhibiting Th17 cell differentiation. It is also known to have anti-apoptotic effects, which is one of the reasons why it has been a hot topic in recent research. Overall, IFN-γ plays a vital role in immune system response and is a key player in maintaining good health.

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