Blood Urea Nitrogen Microplate Assay Kit

BUN

Quantification and Detection of Blood Urea Nitrogen Concentration is an important analytical procedure used to assess kidney function. This procedure involves measuring the amount of blood urea nitrogen (BUN) present in the bloodstream. By determining BUN levels, healthcare professionals can identify and monitor conditions such as kidney disease or dysfunction.
To perform this test, a blood sample is collected and sent to a laboratory where it undergoes analysis. The first step involves the enzymatic cleavage of urea by urease, resulting in the production of ammonia. Ammonia then reacts with alpha-ketoglutarate in the presence of NADH and glutamate dehydrogenase, forming L-glutamate and NAD+. The decrease in NADH concentration is measured spectrophotometrically, and it is directly proportional to the BUN concentration in the sample.
The measurement of BUN content is crucial in assessing kidney function, as higher BUN levels indicate impaired renal function. It helps in diagnosing various kidney disorders such as acute kidney injury, chronic kidney disease, and urinary tract obstruction. Additionally, this test is useful in monitoring the effectiveness of treatments and medications prescribed to manage kidney-related conditions.
It is important to note that factors such as diet, medications, and hydration status can influence BUN levels. Therefore, healthcare providers consider these variables when interpreting test results. In some cases, additional tests may be required to obtain a comprehensive assessment of kidney function.
In conclusion, the detection and quantification of blood urea nitrogen content is a valuable tool for evaluating kidney function. This procedure aids in the diagnosis and monitoring of kidney-related conditions, providing healthcare professionals with important information to guide treatment decisions. Proper interpretation of BUN levels considers various factors to ensure accurate assessment and evaluation of renal health.

0.05 mmol/L - 1 mmol/L

Shipped and store at 4°C for 6 months.