Acetate Kinase Microplate Assay Kit

Acetate kinase, also known as acetic kinase or acetokinase, is an enzyme with the EC number 2.7.2.1. Its primary function is to catalyze the phosphorylation of acetate using ATP as a phosphate donor. This enzymatic reaction yields acetyl phosphate, which is an important intermediate involved in various metabolic pathways.
Acetate kinase plays a vital role in various microorganisms, including bacteria and archaea, as it helps them utilize acetate as a carbon and energy source. The enzyme acts as a key regulator in the acetate metabolism pathway by facilitating the conversion of acetate into acetyl-CoA.
The structural and biochemical properties of acetate kinase have been extensively studied to better understand its catalytic mechanism and physiological significance. It typically consists of a single polypeptide chain that folds into a compact structure, often referred to as a Rossmann fold, which provides the necessary binding sites for ATP and acetate.
Several studies have focused on elucidating the molecular details of how acetate kinase binds and phosphorylates its substrates. These investigations have revealed essential amino acid residues involved in substrate recognition and catalysis. Understanding the enzymatic function of acetate kinase is crucial not only for gaining insights into microbial metabolism but also for potential applications in biotechnology and bioengineering.
In summary, acetate kinase is an enzyme responsible for the phosphorylation of acetate using ATP. It plays a crucial role in microbial metabolism and serves as an essential component in the utilization of acetate as a carbon source. Further research on this enzyme can provide valuable information for various scientific and practical purposes.

One possible rearranged version of the content could be:
Measuring the activity of acetate kinase is a crucial aspect of microbial metabolism research. This enzyme plays a vital role in the conversion of acetate into ATP, a molecule that provides energy for various cellular processes.
To detect acetate kinase activity, scientists commonly use different assay methods, such as spectrophotometry and radioactive labeling. These techniques allow researchers to quantify the amount of ATP produced by the enzyme, which correlates with its activity level.
Furthermore, the detection and quantification of acetate kinase activity can provide insights into the metabolic pathways of different microorganisms, as well as their response to changes in environmental conditions. Hence, it can contribute to developing novel biotechnologies and therapeutic approaches.

4 umol/L - 400 umol/L

Shipped and store at 4°C for 6 months.