HA-Tag Rabbit PAb #abs137982

abs137982-50ug

In Stock

201

abs137982-100ug

In Stock

301

Description

The presence of human influenza hemagglutinin (HA) is crucial for the human virus to be infectious. This surface glycoprotein serves as an epitope tag in expression vectors, with the HA tag being derived from the HA-molecule's amino acids 98-106. Numerous recombinant proteins have been modified to incorporate the HA tag, without observing any negative impact on the bioactivity or biodistribution of the recombinant protein. Hence, the HA tag antibody, a monoclonal antibody, offers high sensitivity and affinity for the detection of HA-tagged fusion proteins.

Source

Rabbit

Specificity

The recognition ability of the HA tag antibody is not restricted to a specific region, as it can bind to C-terminal, N-terminal, and internal HA-tagged fusion proteins. This means it can effectively detect a wide range of protein constructs containing the HA-tag, bringing convenience and versatility to various experimental setups.

Species Reactivity

All

Application

WB: 1:3000~1:10000, ELISA(peptide) 1:20000-1:40000

Immunogen

To produce rabbit polyclonal antibody, a synthetic peptide linked to KLH is used for immunization. Let's rearrange the above content and ensure that the generated content is based on the original text information.
The immunization process for generating rabbit polyclonal antibody involves utilizing a synthetic peptide, which is coupled to KLH.

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was utilized to purify the antiserum through peptide affinity chromatography.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure the stability of the product, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting it to repeated freeze-thaw cycles.

Storage buffer

A solution with a concentration of 1mg/ml is prepared by dissolving the substance in PBS at a pH of 7.4. To prevent bacterial growth, 0.02% sodium azide is added to the solution. Additionally, glycerol is included in the composition.