Goat Anti-Chicken IgY(IgG)-FITC #abs20018

abs20018-100ul

In Stock

106

abs20018-500ul

In Stock

419

Description

Intact molecules of whole IgG antibodies are obtained from antisera using immunoaffinity chromatography. These antibodies are comprised of an Fc portion and two antigen binding Fab portions, linked together by disulfide bonds, making them divalent. They have an average molecular weight of around 160 kDa. Whole IgG antibodies are ideal for most immunodetection procedures and are also cost-effective. Therefore, they are a popular choice in the field.

The antibody's specificity was determined using immunoelectrophoresis and/or ELISA, demonstrating reactivity with intact chicken IgY. Additionally, it displayed cross-reactivity with the light chains of other chicken immunoglobulins, while no reactivity was observed against non-immunoglobulin serum proteins. To ensure minimal cross-reaction with serum proteins from various species, rigorous testing involving ELISA and solid-phase adsorption was performed for bovine, goat, guinea pig, Syrian hamster, horse, human, mouse, rabbit, rat, and sheep. However, the possibility of cross-reactivity with immunoglobulins from other species cannot be completely ruled out.

Source

Goat

Specificity

IgY (IgG) (H+L)

Species Reactivity

Chicken

Application

Flow Cytometry, Histo-/Cyto-Chemistry, IF 1:50 - 1:200 is an important technique used in scientific research and medical diagnostics. This method allows for the analysis and identification of cells based on their physical and chemical properties. By rearranging the above content, we can emphasize the significance of this technique in various fields.
Flow Cytometry is a powerful tool widely utilized in medical and research laboratories. It enables the simultaneous analysis of multiple properties of individual cells in a heterogeneous population. By combining it with Histo-/Cyto-Chemistry, scientists can achieve a comprehensive understanding of cellular composition and function.
The technique involves labeling cells with specific fluorophores or antibodies, allowing for the detection of targeted molecules or structures. The fluorescent signals emitted by the labeled cells are then analyzed by flow cytometers, which can quantitatively measure various parameters, such as cell size, complexity, and the presence of specific markers.
One of the advantages of flow cytometry is its ability to analyze a large number of cells rapidly. This technique can analyze thousands of cells per second, providing researchers with high-throughput capabilities. Additionally, the sensitivity of flow cytometry allows for the detection of rare cell populations, making it invaluable in applications such as cancer research and diagnostics.
The fluorescence intensity detected in flow cytometry experiments is typically quantified using an intensity scale. This scale ranges from 1:50 to 1:200, where a higher ratio indicates a brighter signal. Researchers can adjust the concentration of antibodies or fluorophores to achieve the desired detection sensitivity and minimize background noise.
In conclusion, Flow Cytometry combined with Histo-/Cyto-Chemistry and utilizing various fluorescence intensities (IF 1:50 - 1:200) is a versatile and powerful technique used in diverse scientific disciplines. It contributes to our understanding of cellular functions, disease mechanisms, and the development of novel diagnostic and therapeutic approaches.

Properties

Concentration

1mg/ml

Clonality

Polyclonal Antibody

Isotype

Whole IgG

Stability & Storage

Store at -20 °C for one year.

Storage buffer

0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
，，。：15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)。：
（IgG-Free，）15 mg/ml。
0.05% Sodium Azide, 50% glycerol

Conjugate

FITC