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Rabbit Anti-TUBA4A Polyclonal Antibody#abs135601

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Description

Catalog-specification	Delivery time	USD price
abs135601-50ug	1-2 Weeks	201.0
abs135601-100ug	1-2 Weeks	301.0

Overview
catalog	abs135601
Description	Eukaryotic microtubules have a crucial role to play in various functions and are composed of alpha and beta tubulin heterodimers. The genes responsible for encoding these elements belong to one of the six different tubulin superfamily families. The alpha, beta, and gamma tubulin genes can be found in all eukaryotes. The alpha and beta tubulins are the primary components of microtubules, while the gamma tubulin is essential in the nucleation of microtubule assembly. There are several genes for both the alpha and beta tubulin, and they exhibit high conservation among and between species. This gene encodes an alpha tubulin which is a greatly conserved homologue of the rat-specific alpha tubulin found in the testis. These findings are in accordance with RefSeq.
Other names	The alpha-tubulin 1 protein goes by various names, including ALS22, B ALPHA 1, and TUBA1A. It is also known as testis-specific alpha-tubulin and has been labeled with numerous other aliases such as Hum a tub1, Hum a tub2, and Tubulin H2-alpha. Additionally, the protein has been referred to as Tubulin alpha, Tubulin alpha-1 chain, and Tubulin, alpha 1 (testis specific). Other names for the protein include MGC171407, MGC55332, bA408E5.3, and LIS3. It is also known as Tuba4a and has been labeled as tubulin alpha-1B chain and Tubulin alpha-4A chain. The protein has also been identified as tubulin, alpha 1, like and is categorized under Tubulin, alpha, testis-specific. Overall, the numerous aliases of alpha-tubulin 1 highlight its complex nature and reflect the diverse range of roles that the protein plays in various biological processes. Its presence is essential for proper cellular function and helps to maintain the structural integrity of cells.
Source	Rabbit
Specificity	The antibody for TUBA4A can detect total TUBA4A levels within the body. It is designed specifically to recognize endogenous levels of the protein. There are no artificial sources of TUBA4A that it can detect, ensuring accurate and reliable results. By using this antibody, researchers can accurately measure the levels of TUBA4A within an organism or system, enabling greater understanding of its functions and interactions with other proteins.
Species Reactivity	Human;Mouse;Rat
Antigen	TUBA4A
Application	The recommended dilutions for WB are between 1:500 to 1:2000, while for IHC the dilutions should be within the range of 1:50 to 1:200. As for ELISA, the suggested dilutions for peptide should be around 1:20000 to 1:40000. It is important to follow these dilutions in order to achieve accurate and reliable results in your experiments.
Immunogen	A synthesized peptide derived from human TUBA4A.
MW	55kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Posttranslational modification	Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly.Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability.
Celluar localization	Cytoskeleton;Cytosol;Extracellular region or secreted;
UniPort	P68366