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Rabbit Anti-TUBA1B Polyclonal Antibody#abs137472

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Description

Catalog-specification	Delivery time	USD price
abs137472-100ug	1-2 Weeks	301.0
abs137472-50ug	1-2 Weeks	201.0

Overview
catalog	abs137472
Other names	The alpha tubulin protein is found throughout the human body and is known by many names including Alpha-tubulin ubiquitous, K alpha 1, TBA1B_HUMAN, and Tubulin alpha 1B. This essential protein plays a vital role in cell division, forming the microtubules that help move chromosomes during mitosis. The Tubulin alpha 1B protein is produced by the TUBA1B gene and is one of the most studied alpha tubulin proteins. Its functions include regulation of cell shape and motility, as well as the maintenance of the cytoskeleton. With its widespread presence and essential role in cell biology, alpha tubulin is a subject of much research and scientific inquiry.
Source	Rabbit
Specificity	The TUBA1B Antibody is capable of detecting the total levels of endogenous TUBA1B in the sample. By using this antibody, researchers can accurately monitor the abundance of TUBA1B protein in their experimental system. It is vital information for studying its diverse biological functions.
Species Reactivity	Human;Mouse;Rat
Antigen	TUBA1B
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human TUBA1B.
MW	50 kDa
Properties
Concentration	1mg/ml
purification	We applied SulfoLink™ Coupling Resin for peptide affinity chromatography in order to purify the antiserum. Let me provide you with some highly similar content by reorganizing the information from the original text.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the product's quality, it is advised to store it at -20 °C for a duration of one year. It is crucial to avoid subjecting it to repeated freeze and thaw cycles.
Storage buffer	This product contains Rabbit IgG dissolved in a buffer solution consisting of phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. It is recommended to store this solution at -20°C, and it can remain stable for up to 12 months from the date of receipt. Please note that the above information is based on the original text and has been rearranged for clarity and coherence.
Target
Background	Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Posttranslational modification	Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly.Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability.Nitration of Tyr-451 is irreversible and interferes with normal dynein intracellular distribution.Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.Tubulin alpha-1B chain: Tyrosination promotes microtubule interaction with CAP-Gly domain-containing proteins such as CLIP1, CLIP2 and DCTN1 (By similarity). Tyrosination regulates the initiation of dynein-dynactin motility via interaction with DCTN1, which brings the dynein-dynactin complex into contact with microtubules (PubMed:26972003). In neurons, tyrosinated tubulins mediate the initiation of retrograde vesicle transport (By similarity).Detyrosinated tubulin alpha-1B chain: Detyrosination is involved in metaphase plate congression by guiding chromosomes during mitosis: detyrosination promotes interaction with CENPE, promoting pole-proximal transport of chromosomes toward the equator (PubMed:25908662). Detyrosination increases microtubules-dependent mechanotransduction in dystrophic cardiac and skeletal muscle. In cardiomyocytes, detyrosinated microtubules are required to resist to contractile compression during contraction: detyrosination promotes association with desmin (DES) at force-generating sarcomeres, leading to buckled microtubules and mechanical resistance to contraction (By similarity).
Celluar localization	Cytoskeleton;Extracellular region or secreted;
UniPort	P68363