Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-TRI31 Polyclonal Antibody#abs132842

Please note that the price provided is only for reference. For detailed pricing information, please get in touch with our sales representative, Vecent. Western blot analysis of TRI31 expression in HEK293 cells ,The lane on the left is treated with the antigen-specific peptide. This product is...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs132842-100ug	1-2 Weeks	301.0
abs132842-50ug	1-2 Weeks	201.0

Please note that the price provided is only for reference. For detailed pricing information, please get in touch with our sales representative, Vecent.

Overview
catalog	abs132842
Other names	The human gene known as C6orf13 codes for a RING protein, which is also referred to as HCG1 or HCGI. This protein has a ring finger domain and is classified as a tripartite motif-containing protein 31, or RNF. Its specific functions are not yet fully understood, but this protein is known to have roles in various cellular processes.
Source	Rabbit
Specificity	Total TRI31 endogenous levels can be detected using the TRI31 antibody. To ensure compliance with your request, I will generate a different version of the content by rearranging the original information.
Species Reactivity	Human
Antigen	TRI31
Application	Here are some alternative versions of the provided information: - To detect your protein of interest by western blot, we recommend using a primary antibody concentration of 1:500 to 1:2000. For immunohistochemistry, a dilution of 1:50 to 1:200 of your primary antibody should work. If you're using an ELISA assay with a peptide substrate, a good starting concentration for your primary antibody is 1:20,000 to 1:40,000. - Our suggested dilutions for primary antibody in different applications are as follows: 1:500 to 1:2000 for western blot, 1:50 to 1:200 for immunohistochemistry, and 1:20,000 to 1:40,000 for ELISA using a peptide substrate. These recommendations have been optimized to provide reliable and consistent results while minimizing background and nonspecific binding. - Depending on the application you are using, we recommend diluting your primary antibody as follows: 1:500 to 1:2000 for western blotting, 1:50 to 1:200 for immunohistochemistry, and 1:20,000 to 1:40,000 for peptide-based ELISA. These concentrations have been tested and shown to produce accurate and reproducible results, but may need to be optimized for specific experimental conditions and samples. - When using our primary antibody in western blot, we suggest diluting it to a concentration of 1:500 to 1:2000 for optimal results. For immunohistochemistry, a dilution of 1:50 to 1:200 should be used. If you're performing an ELISA assay with a peptide substrate, a dilution of 1:20,000 to 1:40,000 for your primary antibody should work well. However, please note that these concentrations may need to be adjusted based on your specific experimental design and conditions.
Immunogen	A synthesized peptide.
Properties
Concentration	1mg/ml
purification	We utilized SulfoLink™ Coupling Resin in the process of peptide affinity chromatography to purify the antiserum. This ensured the purification of the antiserum occurred efficiently and effectively.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure proper storage, store at a temperature of -20 °C for a period of one year. It is important to avoid repeated cycles of freezing and thawing. Please follow these guidelines to maintain the integrity of the product over time.
Storage buffer	7.4 pH，IgG，150mM，0.02%50%。
Target
Background	This protein is likely involved in regulation of anchorage-independent cell growth induced by Src. It is also possible that it possesses E3 ubiquitin-protein ligase activity.
Tissue specificity	Up-regulated in gastric adenocarcinomas.
Posttranslational modification	Auto-ubiquitinated (in vitro).
Celluar localization	Cytosol;Mitochondrion;
UniPort	Q9BZY9