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Rabbit Anti-TPSAB1 Polyclonal Antibody#abs136278

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Description

Catalog-specification	Delivery time	USD price
abs136278-100ug	1-2 Weeks	301.0
abs136278-50ug	1-2 Weeks	201.0

Overview
catalog	abs136278
Description	The family of trypsin-like serine proteases known as tryptases belongs to the peptidase family S1. These enzymes are active only in the form of heparin-stabilized tetramers and are resistant to all known endogenous proteinase inhibitors. The genes that encode tryptases are clustered together on chromosome 16p13.3 and have some unique characteristics. They contain tandem repeat sequences at the 5' flank and 3' UTR that may play a role in regulating mRNA stability, as well as a highly conserved 3' UTR. Additionally, there is an intron upstream of the initiator Met codon, which is not common in other genes. The alleles of this gene exhibit a significant amount of sequence variation, leading some to previously believe that there were separate genes called alpha and beta 1. Beta tryptases are the primary isoenzymes expressed in mast cells, while alpha tryptases are more prevalent in basophils. These enzymes have been linked to the development of asthma and other allergic and inflammatory conditions. As such, tryptases have been studied as potential therapeutic targets for these diseases.
Other names	There are several different types of tryptase enzymes that are produced by mast cells in the body. One such enzyme is alpha II tryptase, which is found in lung tissue and is also known as lung tryptase. Mast cells in the skin produce skin tryptase, while those in the pituitary gland produce pituitary tryptase. Tryptase alpha II and tryptase beta 1 are two other types of tryptase enzymes that are produced by mast cells. Other names for these enzymes include TPS1, TPS2, and MCP 7. Tryptase III is another type of tryptase enzyme that is produced in the body. All of these enzymes are part of the same family of proteases known as tryptases.
Source	Rabbit
Specificity	The TPSAB1 Antibody is designed to specifically recognize and measure the amount of total TPSAB1 protein present in a sample. This antibody is capable of detecting endogenous levels of TPSAB1, allowing researchers to study the expression and regulation of this protein in various biological systems. By rearranging the original content, the key information remains the same, emphasizing the antibody's ability to detect endogenous levels of total TPSAB1 for research purposes.
Species Reactivity	Human;Mouse;Rat
Antigen	TPSAB1
Application	The recommended dilution range for WB is between 1:500 and 1:2000, for IHC it is between 1:50 and 1:200, and for IF/ICC it is between 1:100 and 1:500. For ELISA using a peptide substrate, the recommended dilution range is between 1:20000 and 1:40000. These dilution ranges should be used as a guide and may need to be adjusted based on the specifics of the experiment. It is important to optimize the dilution for each individual experiment to ensure the best possible results.
Immunogen	A synthesized peptide derived from human TPSAB1.
MW	31kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates (PubMed:18854315).
Tissue specificity	Isoform 1 and isoform 2 are expressed in lung, stomach, spleen, heart and skin; in these tissues, isoform 1 is predominant. Isoform 2 is expressed in aorta, spleen, and breast tumor, with highest levels in the endothelial cells of some blood vessels surrounding the aorta, as well as those surrounding the tumor and low levels, if any, in mast cells (at protein level).
Celluar localization	Extracellular region or secreted;
UniPort	Q15661