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Rabbit Anti-TNNT2 Polyclonal Antibody#abs135781

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Description

Catalog-specification	Delivery time	USD price
abs135781-100ug	1-2 Weeks	301.0
abs135781-50ug	1-2 Weeks	201.0

Overview
catalog	abs135781
Description	Troponin is a crucial component that, together with tropomyosin, regulates muscle contraction in response to changes in Ca2+ levels within the cell. It consists of three subunits: troponin C (TnC), which binds to Ca2+; troponin T (TnT), which binds to tropomyosin; and troponin I (TnI), which inhibits muscle contraction. When the heart is stimulated by β-adrenergic signals, TnI (cardiac) is phosphorylated by PKA and PKC at Ser23 and Ser24. This phosphorylation triggers a change in the shape of TnC's regulatory domain, leading to decreased interaction between TnI and TnC. Consequently, the binding affinity of TnC for Ca2+ decreases, resulting in reduced sensitivity of myofilaments to Ca2+ (1-3). Different isoforms of TnT exist in various muscle types: slow skeletal muscle (ssTnT/TNNT1), fast skeletal muscle (fsTnT/TNNT3), and cardiac muscle (cTnT/TNNT2), each potentially having multiple alternatively spliced variants. Detecting the serum levels of cTnT and cTnI through assays has proven valuable in analyzing cardiac injuries.
Other names	Troponin T is a protein found in cardiac muscle, also known as TnTc or cTnT. Mutations in the TNNT2 gene, which codes for cardiac muscle troponin T, have been associated with various types of cardiomyopathy, including dilated cardiomyopathy type 1D (autosomal dominant), hypertrophic cardiomyopathy type 2, LVNC6 and RCM3. Some of the other names for this protein and the associated genes include CMD1D, CMH2, CMPD2, MGC3889 and OTTHUMP00000218095. Overall, troponin T is an important protein involved in regulating muscle contraction in the heart. Its mutations and dysregulation can have significant impacts on heart function, leading to various forms of heart disease. By better understanding the role of troponin T in heart disease, researchers can develop more effective treatments and interventions to improve patient outcomes.
Source	Rabbit
Specificity	The TNNT2 Antibody is capable of detecting total TNNT2 levels that naturally occur in the body. In other words, it can detect TNNT2 proteins that are already present in the system without the need for any additional intervention.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Rabbit;Bovine;Dog;Horse;
Antigen	TNNT2
Application	Here are the revised and rearranged versions of the original text information: - For Western Blot (WB), the recommended dilution range is 1:500 to 1:2000. - Immunohistochemistry (IHC) can be performed using a dilution range of 1:50 to 1:200. - When conducting Immunofluorescence (IF), it is suggested to dilute the sample in the range of 1:100 to 1:500. - For ELISA with peptide antigens, the recommended dilution range is 1:20000 to 1:40000. - Additionally, another ELISA using peptides also suggests a dilution range of 1:20000 to 1:40000.
Immunogen	A synthesized peptide derived from human TNNT2.
MW	36kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Tissue specificity	Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
Posttranslational modification	Phosphorylation at Thr-213 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity.
Celluar localization	Cytoskeleton;Cytosol;
UniPort	P45379