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Rabbit Anti-TLR7 Polyclonal Antibody#abs135693

Please note that the price mentioned above is provided for your reference only. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent. Western blot analysis of Ramos cell lysateusing TLR7 antibody. This product is for research use only, not for use in...

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Description

Catalog-specification	Delivery time	USD price
abs135693-100ug	1-2 Weeks	301.0
abs135693-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is provided for your reference only. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent.

Overview
catalog	abs135693
Description	The Toll-like receptor (TLR) family plays a crucial role in innate immune responses by recognizing various pathogens and triggering defense responses. These receptors are named after their close relative Toll in Drosophila. Upon activation, TLRs associate with cytoplasmic adaptor proteins containing TIR domains, such as MyD88, MAL/TIRAP, TRIF, and TRAM, which lead to the recruitment and activation of IRAK1 and IRAK4. This complex then activates TAK1 and IKK, leading to the degradation of IκB that normally maintains NF-κB inactivity in the cytoplasm. TLR7, 8, and 9 are a group of structurally related TLRs localized to intracellular endosomes, with TLR7 showing the highest expression in lung, placenta, and spleen. TLR7 mediates responses to both synthetic compounds, including imidazoquinolines, and ssRNA viruses, which activate NF-κB and trigger IFN production. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the TIR domain.
Other names	TLR7, also known as Toll-like receptor 7, is encoded by the TLR7 gene in humans. TLR7 belongs to the Toll-like receptor family and plays a crucial role in innate immunity. It is involved in detecting single-stranded RNA viruses and triggering immune responses. The TLR7 protein acts as a pattern recognition receptor and is mainly expressed in immune cells such as dendritic cells and macrophages. Its activation leads to the production of pro-inflammatory cytokines and type I interferons, which are essential for antiviral defense. TLR7 is an important component of the immune system and contributes to the recognition and defense against viral pathogens. Research on TLR7 function and regulation can provide insights into the development of novel therapeutics for viral diseases.
Source	Rabbit
Specificity	The total TLR7 is detected by the TLR7 Antibody, which is capable of identifying the natural levels of TLR7 within an organism. To reiterate, the TLR7 Antibody is specifically designed to detect endogenous levels of total TLR7 protein present in biological samples.
Species Reactivity	Human;Mouse;Rat
Antigen	TLR7
Application	We typically dilute the primary antibody for Western blotting at a range of 1:500 to 1:2000, for immunohistochemistry at a range of 1:50 to 1:200, and for ELISA (peptide) at a range of 1:20000 to 1:40000. Please note that the dilution ratios provided are commonly used, but it is important to optimize the dilutions based on your specific experiment's requirements.
Immunogen	A synthesized peptide derived from human TLR7.
MW	121kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity).
Tissue specificity	Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells.
Celluar localization	Endoplasmic reticulum;Endosome;Golgi apparatus;Lysosome;Plasma Membrane;
UniPort	Q9NYK1