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Rabbit Anti-THOC4 Polyclonal Antibody#abs134966

Please note that the price mentioned above is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent. Western blot analysis of extracts from LOVO cells and K562 cells, using THOC4 antibody. This product is for research use only,...

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Description

Catalog-specification	Delivery time	USD price
abs134966-100ug	1-2 Weeks	301.0
abs134966-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent.

Overview
catalog	abs134966
Other names	ALY/REF, also known as transcriptional coactivator Aly/REF, is an important ally of AML-1 and LEF-1 in transcriptional regulation. It is also referred to as BEF or bZIP-enhancing factor BEF. Functionally, ALY/REF acts as an export factor and coactivator in gene expression. It is a subunit of the THO complex 4, also called Tho4 or THOC4, which has a crucial role in mRNA biogenesis. In humans, the protein encoded by the THOC4 gene plays a vital role in various cellular processes, including transcriptional coactivation.
Source	Rabbit
Specificity	The THOC4 Antibody is specifically designed to detect the total levels of endogenous THOC4. This implies that the antibody is capable of recognizing all forms of THOC4 that exist in the biological system and can therefore be used to study the physiological functions of this protein. To attain accurate and reliable results, it is advisable to follow the recommended protocol for using the THOC4 Antibody in any experimental procedures.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Xenopus;Zebrafish;Pig;Dog;Sheep;Horse;Bovine
Antigen	THOC4
Application	For Western blotting, dilute the primary antibody at a ratio of 1:500~1:1000. For immunofluorescence and immunocytochemistry, use a dilution of 1:100-1:500. For ELISA with peptide, use a dilution of 1:20000-1:40000. It is important to follow the recommended dilutions to ensure accurate and reliable results in your experiments.
Immunogen	A synthesized peptide.
MW	27 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum was purified through the process of peptide affinity chromatography. This method helped purify the antiserum by isolating specific peptides that were linked to the resin.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the product's quality, it is recommended to store it at a temperature of -20 °C for up to one year. To avoid damaging the product, please avoid subjecting it to repeated cycles of freezing and thawing.
Storage buffer	This product contains Rabbit IgG in a phosphate buffered saline solution with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at -20 °C and remains stable for up to 12 months from the date of receipt.
Target
Background	Export adapter involved in nuclear export of spliced and unspliced mRNA. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NFX1 pathway). Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm. TREX recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production; ALYREF/THOC4 mediates the recruitment of the TREX complex to the intronless viral mRNA. Required for TREX complex assembly and for linking DDX39B to the cap-binding complex (CBC). In conjunction with THOC5 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in the nuclear export of intronless mRNA; proposed to be recruited to intronless mRNA by ATP-bound DDX39B. Involved in transcription elongation and genome stability.
Tissue specificity	Expressed in a wide variety of cancer types.
Posttranslational modification	Arg-204 is dimethylated, probably to asymmetric dimethylarginine. Arginine methylation reduces RNA binding.Citrullinated by PADI4.
Celluar localization	Cytosol;Extracellular region or secreted;Nucleus;
UniPort	Q86V81