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Rabbit Anti-TGFA Polyclonal Antibody#abs135619

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Description

Catalog-specification	Delivery time	USD price
abs135619-100ug	1-2 Weeks	301.0
abs135619-50ug	1-2 Weeks	201.0

Overview
catalog	abs135619
Description	The epidermal growth factor (EGF) family comprises transforming growth factor alpha (TGF-alpha), which shares the same receptor as EGFR, and regulates cell proliferation, differentiation and survival (1). All EGF family members have an EGF-like domain that contains six regularly spaced cysteines, and this is conserved across 45-60 amino acids. This domain forms a receptor-binding domain with three disulfide bonds. TGF-alpha was first discovered in retrovirally transformed fibroblasts and is named after its ability to transform cultured fibroblasts (2). However, it was later discovered that TGF-beta is required to fully induce transformation in cells through the activity of TGF-alpha, which potentiates it through a separate receptor (3). The membrane-bound precursor of TGF-alpha, pro-TGF-alpha, can still bind and activate EGFR, even though the soluble TGF-alpha is released after proteolytic cleavage (4). Binding of TGF-alpha or its membrane-bound form to EGFR triggers receptor dimerization and activation of downstream signaling molecules through tyrosine autophosphorylation. TGF-alpha and related peptides are crucial in the progression of cancer and neuropathological processes (5,6).
Other names	Transforming growth factor alpha, also known as TGF alpha, TFGA, EGF-like TGF, ETGF, or Wa1, is a protein that is essential for embryonic development, tissue repair, and cell proliferation. This growth factor acts as a ligand to activate the epidermal growth factor receptor (EGFR) signaling pathway, leading to cellular growth and differentiation. TGF alpha belongs to the EGF-like family of growth factors, which are characterized by the presence of an EGF-like domain in their structure. The EGF-like domain is a conserved sequence of amino acids that forms a loop stabilized by disulfide bonds. TGF alpha also contains six cysteine residues that form three disulfide bonds, which contribute to its stability and biological activity. TGF alpha is synthesized as a precursor protein that undergoes proteolytic cleavage to produce the mature form. The mature form is a soluble protein that can bind to the EGFR and initiate downstream signaling events. In addition to its role in development and tissue repair, TGF alpha has been implicated in the progression of various cancers, including breast, lung, and pancreatic cancer. Overall, TGF alpha is a crucial growth factor that plays a vital role in regulating cellular growth and differentiation during development and tissue repair. Its involvement in cancer highlights the importance of understanding its mechanisms of action, which may provide potential targets for therapeutic intervention.
Source	Rabbit
Specificity	The endogenous levels of total TGFA can be detected by the TGFA Antibody. This antibody is specifically designed to recognize and bind to total TGFA. By detecting the endogenous levels of total TGFA, researchers can gain valuable insights into its expression and function in biological systems. This information is crucial for understanding the role of TGFA in various physiological and pathological processes. Overall, the TGFA Antibody provides a valuable tool for investigating the presence and quantity of total TGFA in samples of interest.
Species Reactivity	Human;Mouse;Rat
Antigen	TGFA
Application	For Western blotting, dilute the primary antibody at a range of 1:500 to 1:2000. When performing immunohistochemistry, the primary antibody should be diluted from 1:50 to 1:200. For immunofluorescence/immunocytochemistry, the recommended primary antibody dilution is 1:100 to 1:500. Finally, if conducting an ELISA assay using a peptide, the primary antibody should be diluted between 1:20000 to 1:40000. These dilution ranges have been determined based on the original text information to ensure optimal results for each experimental technique.
Immunogen	A synthesized peptide derived from human TGFA.
MW	17kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
Tissue specificity	Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines.
Celluar localization	Endoplasmic reticulum;Extracellular region or secreted;Golgi apparatus;Plasma Membrane;
UniPort	P01135