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Rabbit Anti-TCF7 Polyclonal Antibody#abs133470

Description

Catalog-specification	Delivery time	USD price
abs133470-100ug	1-2 Weeks	301.0
abs133470-50ug	1-2 Weeks	201.0

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Overview
catalog	abs133470
Other names	The gene codes for a transcription factor known as T-cell-specific transcription factor 1 or TCF-1, also referred to as TCF7. It plays a crucial role in the development and functioning of T-cells. TCF-1 is a transcriptional regulator, which means that it controls gene expression, specifically in T-cells. The protein is involved in the regulation of various processes such as cell growth, differentiation, and apoptosis. TCF-1 is a member of the high mobility group (HMG) box family of proteins and binds to DNA to activate or suppress the transcription of target genes. In summary, TCF-1 is a critical player in the immune system and its dysregulation is linked to several diseases, including cancer and autoimmune disorders.
Source	Rabbit
Specificity	Endogenous levels of total TCF7 can be reliably detected using the TCF7 Antibody. This antibody specifically targets and recognizes the endogenous form of TCF7, allowing for accurate measurement of its expression levels. By utilizing the TCF7 Antibody, researchers can gain valuable insights into the role and regulation of TCF7 in various biological processes.
Species Reactivity	Human;Mouse
Predictive reaction species	Pig;Bovine;Dog
Antigen	TCF7
Application	Possible rearrangement: For Western blotting, the recommended dilution range is 1:500 to 1:1000. When performing immunofluorescence or immunocytochemistry, we suggest using a dilution of 1:100 to 1:500. For ELISA using a peptide as antigen, the optimal working dilution for the primary antibody is 1:20000 to 1:40000. To recap, the appropriate dilutions for different applications of this antibody are as follows: 1:500-1:1000 for WB, 1:100-1:500 for IF/ICC, and 1:20000-1:40000 for ELISA with peptide antigen. We hope this information helps in your experiments.
Immunogen	A synthesized peptide.
MW	42 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum was purified through peptide affinity chromatography. To ensure the highest quality, this process was carefully conducted to produce a highly concentrated and effective antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure maximum shelf-life, it is recommended that the product be stored at a temperature of -20 °C for a period of one year. It is important to note that repeated freeze/thaw cycles should be avoided in order to maintain the product's integrity. Taking these precautions will help to ensure that the product remains effective and functional throughout its intended lifespan.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4+ CD8+ immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.
Tissue specificity	Predominantly expressed in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium.
Celluar localization	Nucleus;
UniPort	P36402