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Rabbit Anti-STK4 Polyclonal Antibody#abs137168

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Description

Catalog-specification	Delivery time	USD price
abs137168-100ug	1-2 Weeks	301.0
abs137168-50ug	1-2 Weeks	201.0

Overview
catalog	abs137168
Other names	Krs2, also known as Mammalian STE20-like protein kinase 1 (MST1), Mammalian sterile 20 like 1, or Serine/threonine protein kinase Krs 2, is a kinase that responds to stress. It is also referred to as Serine/threonine kinase 4 (STK4), Serine/threonine-protein kinase 4, Serine/threonine-protein kinase Krs-2, STE20-like kinase MST1, or YSK3. The MST1 protein is a member of the sterile 20 (Ste20) family of serine/threonine kinases. It plays a critical role in regulating cell proliferation, differentiation, apoptosis, and immune response. Through its interaction with various proteins, MST1 is involved in a variety of cellular processes, including the regulation of mitochondrial function, cell cycle progression, and cytoskeletal dynamics. Research has also shown that MST1 is involved in the development of various diseases, including cancer, metabolic disorders, and cardiovascular diseases. Therefore, targeting MST1 may provide a potential therapeutic strategy for these diseases. In summary, Krs2/MST1 is a versatile kinase that plays a vital role in regulating various cellular processes and may serve as a target for therapeutic intervention in various diseases.
Source	Rabbit
Specificity	Total STK4 can be detected using the STK4 Antibody, which is capable of recognizing the endogenous levels of the protein. Rearranging the given content, an alternative description can be formed as follows: The endogenous levels of total STK4 can be accurately determined by employing the highly reliable STK4 Antibody.
Species Reactivity	Human
Antigen	STK4
Application	Here are some examples of rearranged and paraphrased sentences based on the original text: - The recommended dilution range for Western blotting is between 1:1000 and 1:3000, while for immunohistochemistry it is between 1:50 and 1:200. For ELISA using a peptide antigen, the suggested dilution is between 1:20000 and 1:40000. - If you are performing Western blot analysis, you should dilute your primary antibody at a ratio of 1:1000 to 1:3000. When staining sections for immunohistochemistry, a dilution range of 1:50 to 1:200 is appropriate. For ELISA experiments using a peptide antigen, the recommended dilution range is 1:20000 to 1:40000. - To obtain optimal results in Western blotting, it is recommended to dilute your antibody at a range of 1:1000 to 1:3000, while for immunohistochemistry, the suggested dilution range is between 1:50 and 1:200. If you are performing an ELISA with a peptide antigen, a dilution of 1:20000 to 1:40000 should be employed. - The dilution range suggested for Western blot analysis is between 1:1000 and 1:3000, while for immunohistochemistry it is between 1:50 and 1:200. A dilution range of 1:20000 to 1:40000 is recommended for ELISA using a peptide antigen. Note: These sentences were generated based on the original text and rephrased to provide different structures that convey the same information.
Immunogen	A synthesized peptide derived from human STK4.
MW	56 kDa
Properties
Concentration	1mg/ml
purification	The SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography in order to purify the antiserum. Let's produce a significantly different content by rearranging the original information, while retaining the same meaning.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. Avoid subjecting the product to multiple freeze and thaw cycles, as this could compromise its stability and effectiveness. Proper storage and handling of the product are crucial to ensure its efficacy and prolong its shelf life.
Storage buffer	This product contains rabbit IgG in a solution of phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Storage should be at -20°C and it is stable for 12 months from the date of receipt.
Target
Background	Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation (By similarity). Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation. Phosphorylates MOBKL1A, MOBKL1B and RASSF2. Phosphorylates TNNI3 (cardiac Tn-I) and alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). Phosphorylates FOXO1 on 'Ser-212' and regulates its activation and stimulates transcription of PMAIP1 in a FOXO1-dependent manner. Phosphorylates SIRT1 and inhibits SIRT1-mediated p53/TP53 deacetylation, thereby promoting p53/TP53 dependent transcription and apoptosis upon DNA damage. Acts as an inhibitor of PKB/AKT1. Phosphorylates AR on 'Ser-650' and suppresses its activity by intersecting with PKB/AKT1 signaling and antagonizing formation of AR-chromatin complexes.
Tissue specificity	Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Ubiquitously expressed.
Posttranslational modification	Autophosphorylated on serine and threonine residues. Phosphorylation at Thr-387 by PKB/AKT1, leads to inhibition of its: kinase activity, nuclear translocation and autophosphorylation at Thr-183. It also diminishes its cleavage by caspases and its ability to phosphorylate FOXO3.Proteolytically cleaved by caspase-3 during apoptosis at Asp-326 and Asp-349 resulting in a 37 kDa or a 39 kDa subunit respectively. The 39 kDa subunit is further cleaved into the 37 kDa form. Proteolytic cleavage results in kinase activation and nuclear translocation of the truncated form (MST1/N). It is less likely that cleavage at Asp-349 is a prerequisite for activation as this site is not conserved in the murine ortholog.
Celluar localization	Cytosol;Nucleus;
UniPort	Q13043