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Rabbit Anti-SPZ1 Polyclonal Antibody#abs138386

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Description

Catalog-specification	Delivery time	USD price
abs138386-100ug	1-2 Weeks	301.0
abs138386-50ug	1-2 Weeks	201.0

Overview
catalog	abs138386
Other names	SPZ1, also known as Spermatogenic leucine zipper protein 1, Testis specific protein 1, and Protein phosphatase 1, regulatory subunit 148, is a transcription factor that belongs to the BHLH Zip family. Other aliases include FLJ25709, MGC133987, NYD TSP1, PP1C gamma, PP1C gamma 2, PPP1R148, and Testis secretory sperm-binding protein Li 232m. This protein is primarily expressed in the testis and is essential for spermatogenesis. SPZ1 is involved in the regulation of gene expression and plays a vital role in the differentiation of germ cells. It interacts with other proteins, such as histone deacetylases, to modulate chromatin structure and function. Understanding the function and regulation of SPZ1 may contribute to the development of novel treatments for male infertility.
Source	Rabbit
Specificity	The SPZ1 Antibody is capable of detecting the presence of total SPZ1 at its natural levels within the body. This antibody can be used to monitor and track levels of SPZ1 in research studies and experiments, providing valuable insights into its role and function in various biological processes.
Species Reactivity	Human;Rat
Antigen	SPZ1
Application	IHC 1:200, WB 1:1000-3000, and ELISA (peptide) 1:20000-1:40000 are common dilutions used in laboratory experiments. These dilutions are crucial for obtaining accurate and reliable results. For Western blotting (WB), the dilution range is typically between 1:1000 and 1:3000, meaning that the primary antibody is diluted by a factor of 1000 to 3000 before application. This ensures that the antibody binds specifically to the target protein on the Western blot membrane. In immunohistochemistry (IHC), the primary antibody is commonly diluted at 1:200, which indicates that it is diluted by a factor of 200. This dilution allows for efficient staining of the target antigen in tissue sections. As for ELISA with peptide antigens, the optimal dilution range is usually between 1:20000 and 1:40000. This ensures that the peptide antigen is appropriately coated onto the ELISA plate and that the antibody binds specifically to the peptide during the detection process.
Immunogen	A synthesized peptide derived from human SPZ1.
MW	133 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent purification via peptide affinity chromatography. In order to produce a similar content in a slightly different format, the original information has been rearranged to reflect the same concept.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is recommended that the item be stored at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the item to frequent cycles of freezing and thawing as this could affect the product's integrity. Please ensure that the item is stored in a suitable environment to maintain its efficacy.
Storage buffer	The storage conditions for Rabbit IgG are as follows: it should be stored at -20 °C in phosphate buffered saline (pH 7.4) with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is important to note that this product is stable for a period of 12 months starting from the date it is received.
Target
Background	Transcription factor that binds to the DNA sequence 5'-CANNTG-3'(E box) and the G-box motif. May play an important role in the regulation of cell proliferation and differentiation during spermatogenesis (By similarity).
Tissue specificity	Specifically and strongly expressed in the testis. Expressed in several tumor cell lines.
Posttranslational modification	Phosphorylated by MAPK1/ERK2 and MAPK3/ERK1.
Celluar localization	Nucleus;
UniPort	Q9BXG8